비소세포폐암에서 microRNA-181a-5p에 의한 당과 지질 대사의 재조정

Alternative Title
Jung Tae Kim
Author(s)
김정태
Alternative Author(s)
Jung Tae Kim
Advisor
임상현
Department
일반대학원 의학과
Publisher
The Graduate School, Ajou University
Publication Year
2020-02
Language
eng
Alternative Abstract
microRNAs play an important role in cancer metabolisms. miRNA-181a-5p has decreased expression, particularly in non small cell lung cancer tissues and mesenchymal like lung cancer cells. Epithelial-mesenchymal transition mechanisms and cancer metabolism are regulated by the same signaling pathway. Functional analysis was performed to identify the functional role of miRNA-181a-5p in cancer metabolism and to find the target gene. To define the role of miRNA-181a-5p in cancer metabolism, we performed LDH assay, glucose uptake assay, Oil Red O staining, and mitochondrial ATP synthase inhibitor assay. The target gene of miRNA-181a-5p was determined by luciferase reporter assay, qRT-PCR, and western blot analyses. The Functional effect of miRNA-181a-5p on cancer metastasis was measured by migration and invasion assays. Experimental results showed that overexpression of miRNA-181a-5p reduced aerobic glycolysis and adipocytes and decreased cancer cell invasion and migration. Expression of SIRT1 and ACSL4 was reduced by the binding of miRNA-181a-5p to the 3’-UTR. These results suggest that miRNA-181a-5p might participate in the reprogramming of cancer metabolism.
URI
https://dspace.ajou.ac.kr/handle/2018.oak/21204
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Graduate School of Ajou University > Department of Medicine > 4. Theses(Ph.D)
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