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비소세포폐암에서 microRNA-181a-5p에 의한 당과 지질 대사의 재조정
- Alternative Title
- Jung Tae Kim
- Author(s)
- 김정태
- Alternative Author(s)
- Jung Tae Kim
- Advisor
- 임상현
- Department
- 일반대학원 의학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2020-02
- Language
- eng
- Alternative Abstract
- microRNAs play an important role in cancer metabolisms. miRNA-181a-5p has decreased expression, particularly in non small cell lung cancer tissues and mesenchymal like lung cancer cells. Epithelial-mesenchymal transition mechanisms and cancer metabolism are regulated by the same signaling pathway. Functional analysis was performed to identify the functional role of miRNA-181a-5p in cancer metabolism and to find the target gene. To define the role of miRNA-181a-5p in cancer metabolism, we performed LDH assay, glucose uptake assay, Oil Red O staining, and mitochondrial ATP synthase inhibitor assay. The target gene of miRNA-181a-5p was determined by luciferase reporter assay, qRT-PCR, and western blot analyses. The Functional effect of miRNA-181a-5p on cancer metastasis was measured by migration and invasion assays. Experimental results showed that overexpression of miRNA-181a-5p reduced aerobic glycolysis and adipocytes and decreased cancer cell invasion and migration. Expression of SIRT1 and ACSL4 was reduced by the binding of miRNA-181a-5p to the 3’-UTR. These results suggest that miRNA-181a-5p might participate in the reprogramming of cancer metabolism.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Medicine > 4. Theses(Ph.D)
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