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비소세포폐암에서 microRNA-181a-5p에 의한 당과 지질 대사의 재조정
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 임상현 | - |
| dc.contributor.author | 김정태 | - |
| dc.date.accessioned | 2022-11-29T03:01:28Z | - |
| dc.date.available | 2022-11-29T03:01:28Z | - |
| dc.date.issued | 2020-02 | - |
| dc.identifier.other | 29522 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/21204 | - |
| dc.description | 학위논문(박사)--아주대학교 의학전문대학원 :의학과,2020. 2 | - |
| dc.description.tableofcontents | I. Introduction 1 A. Introduction 1 1. The relation between Glucose, Lipid metabolism and Cancer cell 1 2. The role of miRNA in glucose and lipid metabolism 1 3. The role of miRNA in Cancer Cell 2 4. miRNA-181a-5p and Non Small Cell Lung Cancer 2 5. Hypothesis 3 II. Subjects 4 A. Materials and Methods 4 1. Cell culture and growth conditions 4 2. Glucose-uptake assay 4 3. Lactate dehydrogenase assay 4 4. Mitochondrial ATP synthase Inhibition assay 4 5. Measurement of extracellular acidification rate (ECAR) after treatment of miRNA-181a-5p 5 6. Lipid Staining 5 7. Invasion and migration assays 5 8. TaqMan miRNA expression assay 6 9. miRNA mimic and siRNA transfection 6 10. qRT-PCR 6 11. Western blot 6 12. Approach to search the potential target genes of miRNA-181a-5p 7 13. Luciferase reporter assays 7 14. Statistical analyses 7 B. Results 8 1. Function of miRNA-181a-5p in aerobic glycolysis 8 2. miRNA-181a-5p reduces total lipid in lung cancer cell 9 3. miRNA-181a-5p inhibits lung cancer cell migration and invasion in vitro 9 4. SIRT1 and ACSL4 transcriptions are repressed by binding of miRNA-181a-5p to the 10 5. Quantitative analysis of miRNA-181a-5p, SIRT1, and ACSL 4 expression in lung cancer cell lines 10 6. miRNA-181a-5p regulates SIRT1 and ACSL4 expression at mRNA and protein levels 11 C. Discussion 11 III. Conclusion 14 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 비소세포폐암에서 microRNA-181a-5p에 의한 당과 지질 대사의 재조정 | - |
| dc.title.alternative | Jung Tae Kim | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Jung Tae Kim | - |
| dc.contributor.department | 일반대학원 의학과 | - |
| dc.date.awarded | 2020. 2 | - |
| dc.description.degree | Doctoral | - |
| dc.identifier.localId | 1134007 | - |
| dc.identifier.uci | I804:41038-000000029522 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000029522 | - |
| dc.description.alternativeAbstract | microRNAs play an important role in cancer metabolisms. miRNA-181a-5p has decreased expression, particularly in non small cell lung cancer tissues and mesenchymal like lung cancer cells. Epithelial-mesenchymal transition mechanisms and cancer metabolism are regulated by the same signaling pathway. Functional analysis was performed to identify the functional role of miRNA-181a-5p in cancer metabolism and to find the target gene. To define the role of miRNA-181a-5p in cancer metabolism, we performed LDH assay, glucose uptake assay, Oil Red O staining, and mitochondrial ATP synthase inhibitor assay. The target gene of miRNA-181a-5p was determined by luciferase reporter assay, qRT-PCR, and western blot analyses. The Functional effect of miRNA-181a-5p on cancer metastasis was measured by migration and invasion assays. Experimental results showed that overexpression of miRNA-181a-5p reduced aerobic glycolysis and adipocytes and decreased cancer cell invasion and migration. Expression of SIRT1 and ACSL4 was reduced by the binding of miRNA-181a-5p to the 3’-UTR. These results suggest that miRNA-181a-5p might participate in the reprogramming of cancer metabolism. | - |
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- Graduate School of Ajou University > Department of Medicine > 4. Theses(Ph.D)
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