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젖산 내성 강화를 위한 류코노스톡 메센테로이데스의 장기간 적응 진화
- Alternative Title
- Hyung Ju Lee
- Author(s)
- Lee, Hyung Ju
- Alternative Author(s)
- Hyung Ju Lee
- Advisor
- 이평천
- Department
- 일반대학원 분자과학기술학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2014-02
- Language
- kor
- Keyword
- adaptive evolution; enhanced tolerance; lactic acid
- Alternative Abstract
- D-lactic acid-producing Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 has been long-term adapted to unfavorable conditions where high concentrations of end-product lactic acid inhibit a balanced growth. Evolving force has been applied into L. mesenteroides ATCC 8293 by growing in MRS media containing increasing lactic acid concentrations of 30 g/L to 95 g/L over 8 months. Of 20 adapted L. mesenteroides mutants, LMA92A, which was isolated on media containing 95 g/L lactic acid, was characterized at systems level. A half maximal inhibitory concentration (IC50) of LMA92A was 33.7 ± 5.1 g/L, which is twice higher than 18.2 ± 2.4 g/L of wild-type (WT). Proteomic analysis of WT and LMA92A shows that expressions of several proteins including chaperonin GroEL and F0F1 ATP synthase subunit beta had been up-regulated in LMA92A. GC/MS analysis of fatty acid compositions of WT and LMA92A shows that concentrations of unsaturated and long-chain fatty acids increased in LMA92A, which help maintain cell membrane integrity. All these positive new traits drove LMA92A to produce 1.6-fold lactic acid titer compared to WT in pH-controlled fed-batch fermentation.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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