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젖산 내성 강화를 위한 류코노스톡 메센테로이데스의 장기간 적응 진화
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이평천 | - |
| dc.contributor.author | Lee, Hyung Ju | - |
| dc.date.accessioned | 2018-11-08T08:17:50Z | - |
| dc.date.available | 2018-11-08T08:17:50Z | - |
| dc.date.issued | 2014-02 | - |
| dc.identifier.other | 16242 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/12518 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2014. 2 | - |
| dc.language.iso | kor | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 젖산 내성 강화를 위한 류코노스톡 메센테로이데스의 장기간 적응 진화 | - |
| dc.title.alternative | Hyung Ju Lee | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Hyung Ju Lee | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2014. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 608215 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000016242 | - |
| dc.subject.keyword | adaptive evolution | - |
| dc.subject.keyword | enhanced tolerance | - |
| dc.subject.keyword | lactic acid | - |
| dc.description.alternativeAbstract | D-lactic acid-producing Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 has been long-term adapted to unfavorable conditions where high concentrations of end-product lactic acid inhibit a balanced growth. Evolving force has been applied into L. mesenteroides ATCC 8293 by growing in MRS media containing increasing lactic acid concentrations of 30 g/L to 95 g/L over 8 months. Of 20 adapted L. mesenteroides mutants, LMA92A, which was isolated on media containing 95 g/L lactic acid, was characterized at systems level. A half maximal inhibitory concentration (IC50) of LMA92A was 33.7 ± 5.1 g/L, which is twice higher than 18.2 ± 2.4 g/L of wild-type (WT). Proteomic analysis of WT and LMA92A shows that expressions of several proteins including chaperonin GroEL and F0F1 ATP synthase subunit beta had been up-regulated in LMA92A. GC/MS analysis of fatty acid compositions of WT and LMA92A shows that concentrations of unsaturated and long-chain fatty acids increased in LMA92A, which help maintain cell membrane integrity. All these positive new traits drove LMA92A to produce 1.6-fold lactic acid titer compared to WT in pH-controlled fed-batch fermentation. | - |
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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