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Reelin Is Necessary For Expression of Cell Adhesion Molecules during Radial Migration
- Alternative Title
- 김진우
- Author(s)
- Kim, Jinwoo
- Alternative Author(s)
- 김진우
- Advisor
- 이영돈
- Department
- 일반대학원 분자과학기술학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2013-02
- Language
- eng
- Alternative Abstract
- Radial glial cells in brain development serve as scaffolds which guide radial migration of cortical neurons. Recent studies show that many kinds of cell adhesion molecules (CAMs) are involved in glial-dependent mode of migration. Reelin plays a key role in neuronal migration and in the positioning of neurons within laminal structures during embryonic corticogenesis. It is also reported that activation of Reelin-Dab1 signaling facilitate the radial glial fiber maturation. In this study, we used neural stem cells (NSCs) culture technique and observed the effect of Reelin-Dab1 signaling on expression of CAMs between radial glial fibers and migrating neurons during radial migration. We observed that neuronal migration guided by RC2-positive radial glial fibers was significantly depressed in Reelin-Dab1 signaling lacking mice (reeler and yotari) than in wild type. During differentiation of NSC from reeler brain, expression level of N-cadherin and integrin α3β1 was lower than in wild type. However, when treated with Reelin to culture medium of wild type and reeler NSC, expression of CAMs was increased in both groups, suggesting that activation of Reelin-Dab1 signaling may facilitate expression of N-cadherin and integrin α3β1 interacting between radial glial fibers and migrating neurons during corticogenesis. In contrast, no difference in astrotactin expression level between wild type and reeler was observed. These results suggest a possibility that Reelin-Dab1 signaling may involve in the neuron-glia interaction for locomotion.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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