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쥐에서 미세구슬주입술을 이용한 만성 고안압증 모델; 폴리우레탄과 폴리메틸메타크릴레이트, 폴리스티렌재질의 비교
- Alternative Title
- Chronic Ocular Hypertensive Model using Microbead Injection in Rats; Comparison of Polyurethane, Polymethylmethacrylate, and Polystyrene
- Author(s)
- 노승수
- Alternative Author(s)
- Seungsoo Rho
- Advisor
- 안재홍
- Department
- 일반대학원 의학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2015-08
- Language
- eng
- Keyword
- Chronic ocular hypertension; Microbead; Axon; Retinal ganglion cell; Intraocular pressure; Polyurethane
- Alternative Abstract
- Purpose: To establish and assess an ocular hypertensive rat model using intracameral injection with various microbeads of different sizes and materials. Methods: Chronic elevation of intraocular pressure (IOP) was induced by injection of various microbeads into the anterior chamber of Sprague-Dawley rat eyes. We compared the IOPs induced by the injection of different microbeads [7- and 17-µm polyurethane (PU), 7- and 15-µm polymethylmethacrylate (PMMA), and 15-µm polystyrene (PS)] and selected the appropriate microbeads for a chronic ocular hypertensive model in terms of IOP elevation and adverse events. IOP changes were observed for 4 weeks after microbead injections. Axonal degeneration was assessed with transmission electron microscopic photographs and RGC loss was assessed with retrograde labeling. Results: Sixty-nine rats were included. Three days after a single injection of microbeads, IOPs were increased by 24.0% by 7-µm PU microbeads, 101.8% by 17-µm PU microbeads, 56.6% by 7-µm PMMA microbeads, 22.0% by 15-µm PMMA microbeads, and 34.7% by 15- µm PS microbeads. 17-µm PU microbeads produced constant IOP elevation with good reproducibility (standard deviation of < 6.6 mmHg). Sustained IOP elevation by two injections of 17-µm PU microbeads resulted in a 42% axon loss and 36.5% RGC loss (p<0.05, Mann-Whitney U test). Conclusions: PU microbead injections offer an applicable and versatile model for a chronic ocular hypertensive model in rats. Among several biomaterials, PU microbeads produced a more stable IOP elevation without adverse events.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Medicine > 4. Theses(Ph.D)
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