cyclic-di-GMP가 H-NS와 Lon을 통해 살모넬라의 병원성관련 유전자들의 발현을 저해시킨다는 내용의 연구

Alternative Title
Jisu Jeong
Alternative Author(s)
Jisu Jeong
일반대학원 분자과학기술학과
The Graduate School, Ajou University
Publication Year
Alternative Abstract
Salmonella enterica serovar Typhimurium injects a set of effector proteins into the cytoplasm of host cells via Salmonella pathogenicity island 1 (SPI-1) type III secretion system (T3SS) and induces bacterial invasion into host cells. Cyclic-di-GMP, a circular RNA dinucleotide synthesized by diguanylate cyclases, functions as a second messenger in a variety of physiological processes, including motility, biofilm formation, and virulence factor production. In this study, in order to address how cyclic-di-GMP regulates virulence factor production in S. Typhimurium, the role of cyclic-di-GMP in SPI-1 regulation was investigated. Overexpression of a diguanylate cyclase AdrA significantly decreased the mRNA expression and protein production of HilD, a master regulator of SPI-1, thereby down-regulating the expression of cognate SPI-1 genes. However, the negative regulation of SPI-1 by cyclic-di-GMP was abolished in the absence of H-NS or Lon, which represses hilD expression and controls HilD turnover, respectively. Considering that AdrA overexpression did not influence hns and lon expression, cyclic-di-GMP might exert negative regulation on SPI-1 by modulating the activity of H-NS and Lon. With regard to a pivotal role of cyclic-di-GMP in controlling motile and sessile lifestyles in diverse bacterial pathogens, this study suggests a new role of cyclic-di-GMP in Salmonella lifestyle conversion between colonization in intestinal lumen and invasion into host cells

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Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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