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Novel type hydrolase인 Bacillus licheniformis로 부터 유래한 BL28의 Characterization, immobilizaion, Crystallization
- Alternative Title
- Hansol Ju
- Author(s)
- 주한솔
- Alternative Author(s)
- Hansol Ju
- Advisor
- 김두헌
- Department
- 일반대학원 분자과학기술학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2014-02
- Language
- eng
- Keyword
- Hydrolase; BL28; Stereoselectivity; CLEA
- Alternative Abstract
- A novel type of hydrolase (BL28) from Bacillus licheniformis was identified, expressed in Escherichia coli, characterized, and immobilized for industrial applications. Biochemical characteristics of BL28 were investigated by performing SDS-PAGE, mass spectrometry, enzyme assays, CD spectroscopy, fluorescence, and in silico analysis. Using fluorescence microscopy, we investigated the effects of several chemical compounds on aggregate formation of BL28. Furthermore, cross-linked enzyme aggregates (CLEAs) of BL28 were prepared and scanning electron microscopy images were obtained. These CLEA-BL28 aggregates exhibited improved catalytic efficiencies and stabilities compared to free BL28 against harsh conditions of thermal or chemical stress. The characteristics of the CLEA-BL28 aggregates highlight their great potential in pharmaceutical and chemical industries.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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