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Xanthomonas axonopodis pv로 부터 새로운 SGNH family member의 동정
- Alternative Title
- Kim Nam Yong
- Author(s)
- 김남용
- Alternative Author(s)
- Kim Nam Yong
- Advisor
- 김 두 헌
- Department
- 일반대학원 분자과학기술학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2014-02
- Language
- eng
- Keyword
- SGNH hydrolase
- Alternative Abstract
- A novel putative lysophospholipase hydrolase (833) from Xanthomonas axonopodis pv was identified, characterized. Sequence analysis of 833 revealed a putative catalytic triad (Ser61, Asp209, and His212), and a conserved sequence motif Ser(S)61-Gly(G)126-Asn(N)128-His(H)212 with moderate identities with other members of the SGNH-hydrolase superfamily. 833 was able to hydrolyze short-chain esters such as p-nitrophenyl acetate (C2), butyrate (C4), and valerate (C5) suggesting that the hydrophobic binding pocket of 833 seems to be optimized to accommodate short acyl chain lengths. 833 displayed its maximal activity at pH 7.0 , 60 °C. Higher thermal and chemical stability of the 833 highlight its potential applicability as a biocatalyst in the pharmaceutical and chemical industries.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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