Development of virus nanoparticles for sensitive antibody detection via genetic and unnatural amino acid modification of the coat proteins

Alternative Title
Development of virus nanoparticles for sensitive antibody detection via genetic and unnatural amino acid modification of the coat proteins
Author(s)
신승건
Alternative Author(s)
seunggun shin
Advisor
유태현
Department
일반대학원 분자과학기술학과
Publisher
The Graduate School, Ajou University
Publication Year
2016-02
Language
eng
Keyword
immuno pcrphage displayantigen detection
Alternative Abstract
The virus particle has attractive features as materials, for example, homogenous size, specific modifications of particle surface via genetic and chemical manipulations, and self-amplification via its own genetic information. These properties make researchers try to develop biomaterials, sensor and scaffolds based on phage. Detection by immuno-PCR exhibits high sensitivity, but it usually requires a complex manufacturing process especially for protein-DNA conjugates. We anticipated that a genetically modified M13 bacteriophage can be used as a detection molecule in immuno-PCR. In this study, we have developed a novel bacteriophage particle which displays Z domain, an IgG binding protein, at the pIII coat proteins. The virus particle has not only a template for PCR but also a binding capability to IgG; thus the new sensing reagent can be used for immune-PCR directly by itself. The cTnI myocardial infarction marker is chosen for the demonstration of an immuno-PCR assay method using the modified bacteriophage. An initially developed immuno-PCR method showed a limit of detection as 0.092 ng cTnI/mL, which is about 4-fold lower than an ELISA method. In order to improve the platform, we are applying the site-specific modification strategies using unnatural amino acids by incorporating the analogues into the pIII and/or pVIII coat proteins. For an example, we are developing a simple method to conjugate IgG to the Z domain-displayed phage particle via a light-mediated reaction.
URI
https://dspace.ajou.ac.kr/handle/2018.oak/12055
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Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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