repository
Inhibitory effect of porcine cartilage-derived extracellular matrix suspension on angiogenesis in vitro
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 민병현 | - |
| dc.contributor.author | Kim, Sun Young | - |
| dc.date.accessioned | 2018-11-08T07:59:28Z | - |
| dc.date.available | 2018-11-08T07:59:28Z | - |
| dc.date.issued | 2013-02 | - |
| dc.identifier.other | 14095 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/9490 | - |
| dc.description | 학위논문(석사)아주대학교 일반대학원 :분자과학기술학과,2013. 2 | - |
| dc.description.tableofcontents | Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 4 1. Preparation of PCP-ws 4 2. Biochemical analysis of PCP-ws 5 3. Cell culture 8 4. Cytotoxicity test 9 5. In vitro anti-angiogenesis assay 10 III. RESULTS 12 1. Properties of PCP-ws 12 2. Cellular toxicity of PCP-ws 16 3. In vitro anti-angiogenesis study 18 IV. DISCUSSION 24 Ⅴ. CONCLUSION 27 REFERENCES 28 국문요약 32 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Inhibitory effect of porcine cartilage-derived extracellular matrix suspension on angiogenesis in vitro | - |
| dc.title.alternative | 김선영 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | 김선영 | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2013. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 570844 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000014095 | - |
| dc.subject.keyword | Porcine cartilage ECM derived | - |
| dc.subject.keyword | water suspension | - |
| dc.subject.keyword | Anti-angiogenesis | - |
| dc.description.alternativeAbstract | Background and Object: Articular cartilage is known as an avascular tissue, which has per se anti-angiogenic factors. Several molecules in cartilage extracellular matrix (ECM) are reported to exhibit antiangiogenic activity, some of which already went through clinical trial for harmaceutical purpose such as chondromodulin-I and type XVIII-derived endostatin. We have found previously that the natural cartilage ECM could be a promising treatment with anti-angiogenic activity. However, wide application of drug necessitates various formula especially in soluble phase. We fabricated evenly distributed water suspension-form biomaterial using the cartilage ECM that could be easily deliverable into the body. In this study, we evaluated whether a water-soluble form of the porcine cartilage-derived ECM biomaterial (PCP-ws, porcine cartilage powder – water suspension) still possesses antiangiogenic property. Materials and Methods: The slices of cartilage tissues obtained from 6-weeks-old porcine knees were lyophilized and comminuted into a particulate form, which was then decellularized by hypotonic buffer and DNase-I treatments. The decellularized ECM powder was solubilized by pepsin/HCl. The toxicity of the PCP-ws was evaluated on mouse fibroblast cell line (L929), rabbit chondrocytes and human umbilical vein endothelial cells (HUVEC) by MTT assay. The effect of PCP-ws on the function of endothelial cells was evaluated including cell adhesion, migration, proliferation and tube formation ability were performed. Results: In this study, it is demonstrated that the porcine cartilage powder (PCP) was successfully decellularized and made to a suspension form (PCP-ws) for easily deliverable biomaterial of anti-angiogenic therapy. The viability of L929 and chondrocyte were slightly decreased. In contrast, the viability of HUVEC was severely decreased by PCP-ws in a dose dependent. PCP-ws was prove to be an excellent substrates for inhibiting endothelial cell adhesion, migration and proliferation, particularly for suppression formation of tube-like structure in vitro. In the tube formation assay, the formation of tube network of HUVECs was significantly inhibited by PCP-ws in a dose-dependent manner. Remarkably, the experimental group treated with 5 mg/ml of PCP-ws showed almost no tube-like structure. The length of tube structure decreased approximately by 17 folds, when compared with the untreated group. Discussion and Conclusion: In this study, it is demonstrated that the porcine cartilage powder (PCP) was successfully decellularized and made to a water suspension form (PCPws) for easily deliverable biomaterial of anti-angiogenic therapy. PCP-ws was prove to be an excellent substrates for inhibiting endothelial cell adhesion, migration and proliferation, particularly for suppression formation of tube-like structure in vitro. Supposedly, PCP-ws have a variety of bioactive factors such as chondromodulin-I, endostatin and sulfated glycosaminoglycan side chains that are known to exist in the articular cartilage. Therefore, these results suggest that PCP-ws has an inhibitory effect on angiogenesis in vitro and could be a useful biomaterial for many applications. | - |
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
-
- License
- STATISTICS
- Total Visit :3,739,944
- Total Download :1,818
- Today View :17,602
