repository
Reelin 쥐에서 Entorhino-Hippocampal Projections 의 이상과 Tau 단백질 인산화와의 연관성 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이영돈 | - |
| dc.contributor.author | Yoon, Yeo-Cho | - |
| dc.date.accessioned | 2018-11-08T07:53:36Z | - |
| dc.date.available | 2018-11-08T07:53:36Z | - |
| dc.date.issued | 2010-02 | - |
| dc.identifier.other | 10660 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/8498 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2010. 2 | - |
| dc.description.abstract | 신경세포에 존재하는 Tau는 미세소관과 결합하는 단백질이다. Tau의 주요 기능은 단백질 내 여러 serine 또는 threonine 위치의 인산화와 탈인산화의 균형 조절을 통하여 미세소관의 안정성을 조절한다. 본 연구에서는 reeler에서 나타나는 내후각뇌피질-해마투사로(Entorhino-hippocampal projection) 이상이 Tau의 비정상적인 인산화와 서로 관련되어 있는지에 대하여 살펴보았다. 그 결과, E18.5 시기의 해마이행부 지역에서 Tau의 여러 인산화 위치 중, Ser202/Thr205(AT8), Ser214/Thr212(AT100), Thr181(AT270), Ser396 (pS396), 그리고 Ser404(pS404) 위치에서 Tau의 인산화는 매우 증가 되었다. 또한, Reeler의 같은 시기에서 Ser202/Thr205(AT8), Ser214/Thr212 (AT100) 인산화 위치에서의 Tau의 인산화는 백색층 지역에서 매우 증가됨을 관찰하였다. 이와는 대조적으로, Ser235/Thr231(AT180) 인산화 위치에서의 Tau의 인산 화는 reeler 의 이 두 지역 모두에서 발견되지 않았다. 특히 별아교세포로 추정되는 GFAP-양성세포는 reeler에서 Tau 인산화가 증가된 지역인 해마이행부와 백색층 지역 에서 증가되었다. 따라서, 내후각내피질로부터 해마로 들어오는 신경섬유는 해마이행부 지역에서 Tau의 비정상적인 인산화에 의하여, 신경섬유의 잘못된 형성을 유도하며, 형성 지연에 영향을 미치는 것으로 추정된다. 또한GFAP-양성세포인 별아교세포는, Tau의 비정상적인 인산화와 관련되는 것으로 생각되어진다. | - |
| dc.description.tableofcontents | ⅰ. ABSTRACT ⅱ.CONTENTS ⅴ. LIST OF FIGURES ⅶ. LIST OF TABLES ⅷ. LIST OF ABBREVIATIONS I. INTRODUCTION 1. Tau protein 2. Reelin 3. Entorhino-hippocampal projections 4. Purpose II. MATERIALS AND METHODS A. MATERIALS 1. Animals 2. Reagents B. METHODS 1. Genotyping 2. Cresyl-violet staining 3. Immunohistochemistry 4. Western blot III. RESULTS 1. Verification of reeler mouse genotype by polymerase chain reaction (PCR) 2. The increase of AT8 (Ser202/Thr205)-positive fibers in hippocampus of reeler, during the developmental stage 3. The increase of AT100 (Ser214/Thr212)-positive fibers in hippocampus of reeler 4. The increase of AT180 (Ser235/Thr231)-positive fibers in hippocampus of reeler 5. The increase of the AT270 (Thr181)-positive fibers in hippocampus of reeler 6. The increase of pS396 (Ser396)-immunoreactivities in hippocampus of reeler 7. The increase of pS404 (Ser404)-positive fibers in hippocampus of reeler 8. Western blot data showing the increase of tau phosphorylation in hippocampus and entorhinal cortex of reeler mice 9. The abnormal lamination of cells and distribution of nerve fibers in subiculum of reeler 10. The accumulation of GFAP-positive cells in subiculum and alveus at E18.5 IV. DISCUSSION V. CONCLUSION VI. REFERENCE 국문요약 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Reelin 쥐에서 Entorhino-Hippocampal Projections 의 이상과 Tau 단백질 인산화와의 연관성 연구 | - |
| dc.title.alternative | Malformation of Entorhino-Hipppocampal Projections Is Associated with Abnormal Phosphorylation of Tau in a Reelin-Deficient Mice, Reeler | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Yeo-Cho Yoon | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2010. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 568594 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010660 | - |
| dc.subject.keyword | tau phosphorylation | - |
| dc.subject.keyword | reeler | - |
| dc.subject.keyword | entorhino-hippocampal projections | - |
| dc.description.alternativeAbstract | Tau, a neuronal specific microtubule-associated protein (MAP), plays an important role in the development of neuronal polarity and projections. The main functions of tau proteins are to modulate the stability of axonal microtubules through dynamic phosphorylation. This study was investigated whether the abnormal tau phosphorylation is associated with the malformation of entorhino-hippocampal projections, one of the major inputs of cortex to formation of hippocampus in reeler mice. At E18.5, phosphorylation at Ser202/Thr205, Ser214/Thr212, Thr181, Ser396, and Ser404 of tau protein in reeler mice was highly increased in the region of subiculum. In addition, at the same stage, phosphorylation at Ser202/Thr205, Ser214/Thr212 of tau in reeler mice was highly increased in the alveus. In contrast, phosphorylation at Ser235/Thr231 was not detected in both regions of reeler mice. At postnatal stage, distribution of immunoreactivity at several sites in hippocampus showed that entering of entorhinal afferents into CA2 and 3 regions in reeler seem to be more delayed than in wild-type. Interestingly, GFAP-positive astrocytes were found in both subiculum and alveus of the reeler. Hence, we suggest that increased tau phosphorylation may induce to malformation of entorhino-hippocampal projection and that GFAP-positive astrocytes may be associated with abnormal tau phosphorylation. | - |
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
-
- License
- STATISTICS
- Total Visit :3,768,788
- Total Download :1,819
- Today View :5,289
