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The Role of Jak3 in Skin Wound Healing
- Alternative Title
- 피부 상처 치유에서 Jak3의 역할
- Author(s)
- 조원태
- Alternative Author(s)
- Jo Won Tae
- Advisor
- 백은주
- Department
- 일반대학원 의학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2023-08
- Language
- eng
- Keyword
- Jak3; fibroblast; sebaceous gland; skin; skin wound healing model
- Alternative Abstract
- Janus kinase 3(Jak3) regulates the proliferation, differentiation, and migration of the immune system and progenitors, and controls organ morphogenesis. Jak3 has been shown as a beneficial target for the treatment of chronic skin inflammatory diseases such as psoriasis and alopecia areata. However, the detailed roles of Jak3 on the skin is not known. Therefore, this study attempted to study the function of Jak3 in the process of healing skin wounds by administering WHIp-131, a selective Jak3 inhibitor, to mouse skin wounds. To find out the role of Jak3 signaling on the skin repair and regeneration, in vivo skin wound healing model on the mouse back skin was conducted. The wound closure had occurred due to pure healing and another contraction capacity. The skin contracture is due to activated fibroblasts and myoblasts. Jak3 inhibitor significantly reduced the skin contraction rather than the healing. In addition, the thickness of the epidermis of the wound bed and the thickness of the dermal muscle layer were decreased by Jak3 inhibition. Fibroblasts from the dermis were cultured to investigate the role of fibroblasts in wound healing and contraction. As a result of administration of Jak3 inhibitor to the in vitro cultured dermal fibroblasts, a significant decrease in proliferation and migration was observed, and it was also confirmed that structure of vimentin did not exist near the nucleus but only at the end of fibroblasts, affecting motility. In addition, it was possible to confirm that isolectin B4 was used as a marker for sebaceous cells, and as a result of observing in vivo and in vitro sebaceous cells, Jak3 inhibition significantly decrease the size of regenerating sebaceous glands, but also the sebocytes. In conclusion, Jak3 signaling is involved in skin contraction and regulates fibroblast proliferation and migration through ytoskeletal changes, including vimentin. It also regulates the area of sebocytes in regenerating pilosebaceous units. In this study, isolectin B4 is suggested as a sebaceous cell marker. Also, it suggests that Jak3 inhibitors have potentials to control the contracture of wounds and to improve hair loss and acne further.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Medicine > 3. Theses(Master)
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