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Various insight on biological research: analysis through bio-data of cells treated with 2′-fucosyllactose, exogenous nitric oxide on the possibility of wound repair, and ovarian protection on chemotherapy
- Author(s)
- 장동민
- Alternative Author(s)
- Dongmin Jang
- Advisor
- 김미란
- Department
- 일반대학원 의생명과학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2023-08
- Language
- eng
- Keyword
- Bio data; GV1001; Nitric oxide
- Alternative Abstract
- Among the human milk oligosaccharides (HMOs), many studies have been conducted on 2-fucosyllactose (2-FL), the most dominant component of HMO, due to its active and non-toxic characteristics. Despite a spotlight on the regulatory effect of 2-FL in immune response, the role of 2-FL in physiological and cellular functions associated with the human skin trouble such as hyperpigmentation has not been elucidated yet. Here, we identified a total of 1,151 DEGs changed by 2-FL in human melanoma cell line (MNT-1) through workflow of our RNA sequencing analysis. Furthermore, comparative transciptome analysis showed that up- and down-stream cellular processes and pathways related to AMPK signaling were also regulated by 2-FL treatment. Thus, we suggest the direction of future research on possibility of 2-FL as a new anti-melanogenic agent for hyperpigmentation through our network model with AMPK signaling. <br> <br>Nitric oxide (NO) is a free radical having an unpaired electron and is a very important molecule in many biological processes including cell proliferation and immune system. However, exogenous nitric oxide (NO) generated through plasma system is not intensively explored on various biological fields such as wound healing. Wound healing is a series of process in which the skin or tissues, repair themselves after injury. Especially, collagen and TGF-beta1 play a major role in tissue remodeling during wound healing. Here, we have manufactured a plasma generating device that can selectively produce nitric oxide (NO), exposed human fibroblast cells (adult and neonatal) to nitric oxide (NO), and showed that the expression of pErk, pSmad3, collagen, and TGF-beta1 were increased after nitric oxide (NO) treatment. However, PD98059, a specific inhibitor of mitogen-activated protein kinase (MEK1), inhibited ERK activated by nitric oxide (NO), and consequently attenuated pSmad3 and collagen. Therefore, we suggest that exogenous nitric oxide (NO) regulates collagen and TGF-beta1 through ERK-SMAD axis. <br> <br>Premature ovarian failure (POF) that could result from chemotherapy applied to young female cancer patients is a significant challenge in reproductive biology. A better understanding on hyperactivation of folliculogenesis or dormant primordial follicles following chemotherapy leading to problem like POF is important to adopt new strategy to maintain fertility in these patient. We provide evidence that supports that GV1001, an immunotherapeutic peptide targeting telomerase, plays a role in the protection of ovarian function through regulation of location of Foxo3a. Here, we showed that anti-VEGFa agent, bevacizumab, induced follicular loss by accelerating primordial follicle growth into primary or secondary follicles concomitant with a decline of serum anti-mullerian hormone (AMH) level and disappearance of Foxo3a signaling in the nucleus as evidenced by immunohistochemical and immunofluorescent assessment. However, GV1001 as a combinatorial administration drug reduced overactivation of folliculogenesis, thereby maintaining ovarian reserve. From this study, we suggest that GV1001 shows potential as a drug protecting from primordial follicle depletion, which may be able to maintain fertility in young cancer patients without hampering the chemical agent. <br> <br>Recently, biological research is changed a method centered on cell or animal experiments into a comprehensive and convergent way of thinking including bioinformatics. Also, it is a trend to obtain more efficient and progressive research results by combining genome analysis and experiments on a wide range of biological or medical problems that are difficult to see or understand experimentally in a large amount of information. In conclusion, we improved research efficiency by using a complex approach such as cell or animal experiment, and bioinformatics. Moreover, based on this approach, it is expected to be used in various fields of biology.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Biomedical Sciences > 4. Theses(Ph.D)
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