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Cartilage Reparability by the Second Microfracture
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | Byoung-Hyun Min | - |
| dc.contributor.author | TRUONG, MINH DUNG | - |
| dc.date.accessioned | 2018-10-16T02:20:05Z | - |
| dc.date.available | 2018-10-16T02:20:05Z | - |
| dc.date.issued | 2010-08 | - |
| dc.identifier.other | 10852 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/2109 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학,2010. 8 | - |
| dc.description.tableofcontents | Table of Contents Abstract i Table of contents iii List of figures iv List of tables v List of abbreviations vi I. Introduction 1 II. Materials and methods 6 1. Experiment design 6 2. Surgical techniques 7 3. Cell culture and colony-forming unit-fibroblasts (CFU-Fs) assay 9 4. Histological evaluation 11 5. Radiological evaluation 12 III. Results 13 1. Surgical techniques 13 2. Colony-forming unit-fibroblasts (CFU-Fs) assay 18 3. Histological evaluation 22 4. Radiological evaluation 25 IV. Discussion 27 V. Conclusion 31 References 32 List of Figures Figure 1. Four types of awl were tested 8 Figure 2. The procedure for CFU-F assay 10 Figure 3. Optimizing of microfracture techniques in rabbit model based on volume of the blood bleeding from microfracture holes 14 Figure 4. Optimizing of microfracture techniques in rabbit model based on micro-CT images of the microfracture holes 15 Figure 5. MF in rabbit model. 16 Figure 6. Hematoxylin and eosin (H&E) ？ stained histological sections of the knee from rabbit sacrificed after microfracture surgery (using 3 mm depth awl) 17 Figure 7. The result of CFU-F test using crystal violet 19 Figure 8. 8 weeks after MF, the healing ability was evaluated by gross image and histological appearances. 23 Figure 9. Safranin-O and H&E staining at 8 weeks after surgery 24 Figure 10. Micro-CT images of the knee from rabbit sacrificed on 8 weeks after the first MF surgery and on 8 weeks after the second MF. 26 List of Tables Table 1. Results of CFU-F test after the first MF and the second MF 21 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Cartilage Reparability by the Second Microfracture | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2010. 8 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 568917 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010852 | - |
| dc.description.alternativeAbstract | -Abstract- Cartilage Reparability by the Second Microfracture Objects: Articular cartilage has some limitations in healing ability. Microfracture (MF) is considered as the first-line treatment for full- thickness cartilage lesion. But in the long-term follow-up after MF, failure rate was increased because MF was influenced by many factors such as capacity of mesenchymal stem cells (MSCs) frequency, clinical factors, loading history and constitution of the patient. Therefore, the aim of this study is to evaluate whether the second MF could stimulate bone marrow effectively in the recurrent cartilage defect lesion after MF. Materials and methods: Sixteen-weeks-old male New Zealand white rabbits were used for this study. In the patellar groove of unilateral knees, the full-thickness chondral defect with 5-mm-diameter and 0.2-mm-depth was created by a biopsy punch. In which, the MF holes were created by using a 3-mm-length awl which was optimized based on the bleeding volume and the defect depth observed with a micro-computed tomography (micro-CT) image. Thirty rabbits were divided into 3 groups. The first group was the negative control group with untreated full-thickness chondral defect. The second group was the control group treated with MF. The third group was the experimental group treated with MF and the second MF again at 8 weeks after the first MF. At the end of 8 weeks, the rabbit in the negative and the control group were sacrificed and the specimens were collected for confirmation healing ability of MF newly developed for this study. The regeneration of new tissues was evaluated based on the histological changes in defect and morphological changes in subchondral bone area by histological staining images and micro-CT images, respectively. The number of colony in colony-forming unit-fibroblasts (CFU-F) test after the re-MF would be compared with the first MF in order to evaluate the number of MSCs derived from bone marrow. Results: MF awl with 3 mm length was a proper surgical tool for present study. At 8 weeks after the first MF, the new bony tissue was observed in subchondral bone plate. Moreover, the average of MSC number was about 120 cells/1 ml bone marrow from the first MF, and there were 150 cells/1 ml bone marrow isolated from second MF. There was no significant difference in the colony formation between the first MF and second MF. The bony repair after the second MF was confirmed. It was observed in subchondral bone plate but it was incomplete. The subchondral bone plate observed at 8 weeks after the second MF was thicker than at 8 weeks after the first MF. Conclusions: Surgical technique and awls designed for this study were well developed and could be widely used for the animal studies about MF. The second MF could be a useful treatment to restore the failed resurfacing of cartilage after MF. Further systematic study will be needed to evaluate the repair of defects made by MF and the differentiation abilities of MSCs came from bone marrow by the second MF. Key Words: Mesenchymal stem cell, Bone marrow stimulation, Second microfracture. | - |
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- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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