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Self-assembled hyaluronic acid nanoparticle as a topical agent for psoriasis treatment
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김욱 | - |
| dc.contributor.author | 권소희 | - |
| dc.date.accessioned | 2022-11-29T02:32:31Z | - |
| dc.date.available | 2022-11-29T02:32:31Z | - |
| dc.date.issued | 2021-02 | - |
| dc.identifier.other | 30547 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/20067 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2021. 2 | - |
| dc.description.tableofcontents | Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS and METHODS 4 1. Materials 4 2. Preparation and characterization of HA-LCA NP 5 3. Cell culture and treatment 6 4. Mice and induction of psoriasis mouse model 7 5. RNA extraction and quantitative real-time polymerase chain reaction (qRT-PCR) analysis 7 6. Cells viability test 8 7. Cell isolation 9 8. FACS analysis 9 9. Western blot 10 10. Franz diffusion cell (FDC) 11 11. Histological analysis 12 12. Statistical analysis 13 Ⅲ. RESULTS 16 1. Synthetic scheme and characteristics of self-assembled HA-LCA NP 16 2. HA-LCA NP inhibits the polarization of resting macrophages into M1 phenotype 20 3. Effects of free HA and LCA on the expression of M1 marker genes in THP-1 cells 26 4. HA-LCA NP inhibits LPS-induced TLR4 signaling pathway in macrophages 31 5. Effects of HA-LCA NP on the expression of psoriasis-related genes in keratinocytes 34 6. Skin-penetrating ability of HA-LCA NP 37 7. Topical treatment of HA-LCA NP alleviates psoriatic symptoms in IMQ-induced psoriasis mouse model 45 8. Topical treatment of HA-LCA NP inhibits keratinocyte hyperproliferation 50 9. Topical treatment of HA-LCA NP decreases infiltration of macrophages into the dermis 53 Ⅳ. DISCUSSION 56 Ⅴ. REFERENCE 60 Ⅵ. ABSTRACT IN KOREAN (국문초록) 65 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Self-assembled hyaluronic acid nanoparticle as a topical agent for psoriasis treatment | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Sohui Kweon | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2021. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 1203405 | - |
| dc.identifier.uci | I804:41038-000000030547 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000030547 | - |
| dc.subject.keyword | psoriasis | - |
| dc.description.alternativeAbstract | Self-assembled hyaluronic acid nanoparticles (HA-NPs) are widely used as drug delivery systems because HA has biocompatible, non-toxic, and non-immunogenic. Recently, drug-free HA-NPs have been shown to exert therapeutic effects in type 2 diabetes and atherosclerosis. Here, I investigate the in vitro and in vivo therapeutic efficacy of an empty HA-NP itself in psoriasis, the most common chronic inflammatory skin disease, after topical treatment via transcutaneous delivery. HA-lithocholic acid (LCA) NPs are amphiphilic compounds composed of hydrophilic HA and hydrophobic LCA that self-assemble in an aqueous environment to form sphere-shaped NPs. HA-LCA NPs inhibited the lipopolysaccharide (LPS)-induced polarization of the human monocyte cell line THP-1 into pro-inflammatory M1 macrophages and reduced the expression of interleukin (IL)-23, a key player in psoriasis and inflammatory cascades. In addition, HA-LCA NPs decreased the expression of pro-inflammatory genes in the human keratinocyte cell line HaCaT stimulated with IL-17 and IL-22. Furthermore, transcutaneous administration of HA-LCA NPs resulted in significant localization into the dermis and epidermis in control and imiquimod (IMQ)-induced psoriasis mouse models, thereby effectively ameliorating psoriasis-like skin inflammation as reflected by reduced skin tissue thickening, inflammation, and cytokine expression. These results suggest that skin-penetrating HA-LCA NPs may serve as novel therapeutic agents for the treatment of human psoriasis-like dermatitis by suppressing the inflammatory response. | - |
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- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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