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Purification of mevalonate from fermentation broth and conversion of biomevalonate into biomevalonolactone
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이평천 | - |
| dc.contributor.author | 윤희찬 | - |
| dc.date.accessioned | 2022-11-29T02:32:18Z | - |
| dc.date.available | 2022-11-29T02:32:18Z | - |
| dc.date.issued | 2020-08 | - |
| dc.identifier.other | 30329 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/19825 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2020. 8 | - |
| dc.description.tableofcontents | 1. Introduction 1 2. Materials and methods 3 2.1 Bacterial strains and plasmids 3 2.2 Media and culture condition 5 2.3 Pilot scale fermentation 5 2.4 MVL purification process 7 2.5 NMR analysis 7 3. Results 8 3.1 MVA production 8 3.2 Bioconversion of MVA into MVL and purification of MVL 8 4. Conclusion 13 5. Reference 14 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Purification of mevalonate from fermentation broth and conversion of biomevalonate into biomevalonolactone | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Heechan Yoon | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2020. 8 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 1151737 | - |
| dc.identifier.uci | I804:41038-000000030329 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000030329 | - |
| dc.subject.keyword | Escherichia coli | - |
| dc.subject.keyword | MVA | - |
| dc.subject.keyword | fermentation | - |
| dc.description.alternativeAbstract | Mevalonate (MVA) is a key compound of living organisms including bacteria, plants, and humans. MVA and mevalonolactone (MVL), a lactonized form of MVA, are important for pharmaceutical, cosmeceutical, and biotechnological applications. Although (R, S)-MVA with 50% enantiomeric purity is mainly produced by chemical synthesis, recently, microbial fermentation processes for MVA production have been considered as an alternative to the chemical synthesis because of high enantiomeric purity [(R)-MVA] and high titer. In the present study, bio-MVA produced by a fermentative process was decolorized by a charcoalbased method and then chemically transformed into bio-MVL without byproducts by means of phosphoric acid as an acid catalyst. The final bio-MVL was (R)-MVL with over 99% enantiomeric purity according to 1H NMR analysis. | - |
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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