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Effect of Tissue Transglutaminase on Steroid Resistance in T-Cell Acute Lymphoblastic Leukemia
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 박준은 | - |
| dc.contributor.author | 정현주 | - |
| dc.date.accessioned | 2022-11-29T02:32:13Z | - |
| dc.date.available | 2022-11-29T02:32:13Z | - |
| dc.date.issued | 2020-08 | - |
| dc.identifier.other | 30197 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/19723 | - |
| dc.description | 학위논문(박사)--아주대학교 일반대학원 :의학과,2020. 8 | - |
| dc.description.tableofcontents | I 서론 (Introduction) 1 II 본론 3 A. 실험방법 (Materials and Methods) 3 1. Cell lines and cell maintenance 3 2. Reagents 3 3. Immunofluorescence analysis 4 4. Immunoblot analysis 4 5. Tissue transglutaminase (TG2) enzymatic activity assay 5 6. Colorimetric cell viability assay 5 7. Flow cytometry 5 8. Nuclear factor-κB (NF-κB) activity assay 6 9. Statistical analysis 6 B. 실험결과 (Results) 7 1. TG2 expression in T-cell acute lymphoblastic leukemia (T-ALL) cells. 7 2. Generation of steroid-adapted (SA) T-ALL cells 9 3. Steroid adaptation affects TG2 and NF-κB activity in T-ALL. 12 4. TG2 inhibition reduces steroid resistance in SA T-ALL. 14 III 결론 (Discussion) 19 참고문헌 23 | - |
| dc.language.iso | kor | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Effect of Tissue Transglutaminase on Steroid Resistance in T-Cell Acute Lymphoblastic Leukemia | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Hyun Joo Jung | - |
| dc.contributor.department | 일반대학원 의학과 | - |
| dc.date.awarded | 2020. 8 | - |
| dc.description.degree | Doctoral | - |
| dc.identifier.localId | 1151634 | - |
| dc.identifier.uci | I804:41038-000000030197 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000030197 | - |
| dc.subject.keyword | T-cell acute lymphoblastic leukemia | - |
| dc.subject.keyword | Tissue transglutaminase | - |
| dc.subject.keyword | steroid resistance | - |
| dc.description.alternativeAbstract | Aim: To improve survival in patients with glucocorticoid-resistant T-cell acute lymphoblastic leukemia (T-ALL), it is critical to develop new therapeutic strategies to overcome steroid resistance. Materials and Methods: Biochemical and molecular methodologies were used to evaluate whether tissue transglutaminase (TG2) confers steroid resistance in T-ALL. Results: T-ALL cells were found to express elevated levels of TG2. We went on to generate models of steroid-adaptedsubclonesofT-ALLcelllinesthatwerenotablylesssensitivetosteroidsthantheparental cells. The steroid-adapted subclones showed increased TG2 expression and nuclear factor-κB (NF-κB) activity compared to T-ALL parental cells. Inhibition of TG2 suppressed steroid resistance and improved steroid cytotoxicity in steroid-adapted subclones of T-ALL in association with reduced NF-κB activity. Conclusion: It is important to note that this study identified that possible changes in TG2 expression may serve as a new target aimed at overcoming steroid resistance in T-ALL. | - |
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- Graduate School of Ajou University > Department of Medicine > 4. Theses(Ph.D)
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