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S-allylcysteine 에 의한 Met의 불활성화는 간세포성장인자에 의해 유도된 비인두암 세포들의 이동과 침습을 저해한다
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 전미선 | - |
| dc.contributor.author | Cho, Oyeon | - |
| dc.date.accessioned | 2019-10-21T07:26:18Z | - |
| dc.date.available | 2019-10-21T07:26:18Z | - |
| dc.date.issued | 2016-02 | - |
| dc.identifier.other | 21366 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/18764 | - |
| dc.description | 학위논문(박사)--아주대학교 일반대학원 :의학과,2016. 2 | - |
| dc.description.tableofcontents | I. INTRODUCTION 1 II. MATERIALS AND METHODS 3 A. MATERIALS 3 1. Reagents 3 B. METHODS 3 1. Cell Culture 3 2. Wound Healing Assay 3 3. Invasion Assay 4 4. MTT Assay 4 5. Soft Agar Colony Forming Assay 4 6. Western Blot Analysis 4 7. Gelatin Zymography 5 8. Statistical Analysis 5 III. RESULTS 6 1. Wound healing and invasion assay 6 2. MTT and soft agar assay 9 3. Western blot and gelatin zymography 11 IV. DISCUSSION 14 V. CONCLUSION 20 REFERENCES 21 국문요약 23 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | S-allylcysteine 에 의한 Met의 불활성화는 간세포성장인자에 의해 유도된 비인두암 세포들의 이동과 침습을 저해한다 | - |
| dc.title.alternative | Met Inactivation by S-allylcysteine Suppresses Migration and Invasion of Nasopharyngeal Cancer Cells Induced by hepatocyte growth factor | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 의학과 | - |
| dc.date.awarded | 2016. 2 | - |
| dc.description.degree | Doctoral | - |
| dc.identifier.localId | 739308 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000021366 | - |
| dc.subject.keyword | Nasopharyngeal cancer | - |
| dc.subject.keyword | Met | - |
| dc.subject.keyword | S-allylcysteine | - |
| dc.subject.keyword | Migration | - |
| dc.subject.keyword | Invasion | - |
| dc.subject.keyword | HNE1 | - |
| dc.subject.keyword | HONE1 | - |
| dc.description.alternativeAbstract | Past studies have reported that S-allylcysteine (SAC) inhibits the migration and invasion of cancer cells through the restoration of E-cadherin, the reduction of matrix metalloproteinase (MMP) and Slug protein expression, and inhibition of the production of reactive oxygen species (ROS). Furthermore, evidence is emerging that shows that ROS induced by radiation could increase Met activation. Following on these reports of SAC and Met, we investigated whether SAC could suppress Met activation. Wound healing, invasion, MTT, soft agar colony forming, western blotting, and gelatin zymography assays were performed in the human nasopharyngeal cancer cell lines HNE1 and HONE1 treated with SAC (0, 10, 20, or 40 mM) and hepatocyte growth factor (HGF). This study showed that SAC could suppress the migration and invasion of HNE1 and HONE1 cell lines by inhibiting p-Met. An increase of migration and invasion induced by HGF and its decrease in a dose dependent manner by SAC in wound healing and invasion assays was observed. The reduction of p-Met by SAC was positively correlated with p-focal adhesion kinase (p-FAK) and p-extracellular related kinase (p-ERK in both cell lines). SAC reduced Slug, MMP 2, and MMP 9 involved in migration and invasion with the inhibition of Met-FAK signaling. These results suggest that SAC inhibited not only Met activation but also the downstream FAK, Slug, and MMP expression. Finally, SAC may be a potent anticancer compound for nasopharyngeal cancer treated with radiotherapy. | - |
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