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Characterization of cathepsin B cysteine protease from Naegleria fowleri excretory-secretory proteins exerting immunomodulatory effects
- Author(s)
- Lee, jinyoung
- Advisor
- 신호준
- Department
- 일반대학원 의생명과학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2015-02
- Language
- eng
- Keyword
- Naegleria fowler; cytokine; MAPK; microglial cell; 파울러자유아메바; 시스테인 단백분해효소
- Alternative Abstract
- .Naegleria fowleri, a free-living amoeba that is found in diverse environmental habitats, can cause an acute fulminating hemorrhagic meningoencephalitis, called primary amoebic meningoencephalitis (PAM) in experimental animals and in humans. Pathogenicity of N. fowleri may be induced by contact-dependent and contact-independent mechanisms, and both mechanisms lead to death of host cells. Several proteins secreted from N. fowleri are likely to be associated with contactindependent pathogenic mechanism of the amoeba. The excretory and secretory proteins of N. fowleri (Nf-ESPs) include phospholipase, proteases, peroxiredoxins and thrombin receptor. However, the precise mechanism induced by Nf-ESPs in PAM is not fully understood yet. In this study, the cytopathic changes and inflammatory responses induced by Nf-ESPs were analyzed in BV2 microglial cells. Several cytokines, particularly IL-1α and TNF- α were highly up-regulated by Nf-ESPs. Also, activation of P38 and JNK was observed in BV2 cells treated with Nf-ESPs. The results collectively suggest that Nf-ESPs may play an important role in N. fowleri mediated-PAM through induction of inflammatory response in microglial cells and activation of MAPKs signal pathway.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Biomedical Sciences > 4. Theses(Ph.D)
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