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Expression of Follicular Helper T-cell Markers in Primary Cutaneous T-cell Lymphoma
- Alternative Title
- 박지혜
- Author(s)
- Park, Ji-Hye
- Alternative Author(s)
- 박지혜
- Advisor
- 김유찬
- Department
- 일반대학원 의학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2013-02
- Language
- eng
- Alternative Abstract
- Background and objectives: A distinct subset of T helper cells, named follicular helper T- (TFH) cells, have been recently described. The TFH-cells can be identified on the basis of the expression of several markers including: the chemokine CXCL13; the costimulatory molecules PD-1 and inducible costimulator (ICOS); and the transcription factor Bcl-6. The TFH-cells appeared to be localized in several subsets of T-cell lymphomas. However, their localization in cutaneous T-cell lymphomas has been rarely described. In this study, the clinical features, histopathological morphology, and expression of TFH markers in cutaneous T-cell lymphomas were investigated. Materials and methods: Forty-nine Korean patients (24 men, 25 women) diagnosed with cutaneous T-cell lymphoma were examined: 25 patients with mycosis fungoides (MF), 9 with lymphomatoid papulosis, 8 with anaplastic large cell lymphoma, 4 with subcutaneous panniculitis-like T-cell lymphoma, 2 with peripheral T-cell lymphoma, and 1 with NK/T-cell lymphoma. Chart review was performed to evaluate the clinical features. Hematoxylin and eosin (H&E) staining was used to study the general histopathological changes. Additionally, immunohistochemical staining for CD10, Bcl-6, ICOS, CXCL13, and PD-1 were performed. The extent of staining was scored from 0 to 3 by 2 independent observers. Results: PD-1 was frequently expressed in most of the MF cases, but was rarely expressed in other cutaneous T-cell lymphomas. Bcl-6, CXCL13, ICOS, and CD10 were scantly expressed in most T-cell lymphomas, including MF. Interestingly, the staining for PD-1 was negative in all the MF cases with large-cell transformation. No correlation was observed among clinical morphology, disease course, and PD-1 expression rate in the MF cases. Conclusions: Most of the MF samples expressed TFH-cell markers. This finding suggests that some MF tumor cells might consist of TFH-cells. In addition, higher PD-1 expression rates in MF than in other cutaneous T-cell lymphomas implies that PD-1 can be an MF-sensitive marker, and it might be useful in differentiating MF from other cutaneous T-cell lymphoma. The loss of PD-1 expression in large-cell–transformed MF suggests that PD-1 downregulation may be related to the processes responsible for large-cell transformation.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Medicine > 3. Theses(Master)
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