The glycinecin R (glyR) is one of bacteriocins produced by Xanthomonas axonopodis pv. glycines 8ra. It shows strong bactericidal activity on number of major crop pathogens including Xanthomonas oryzae pv. oryzae and Xanthomonas campestris pv. vesicatoria. To identify the receptor on the X. campestris pv. vesicatoria 833 responsible for glyR recognition a mutant library was constructed using transposon-insertion mutagenesis, and a mutant X. campestris pv. vesicatoria 833 clone which is resistant to glyR was isolated. The transposon-inserted genomic region was cloned, and the gene inactivated by transposon insertion was identified as the pilA gene by nucleotide sequence analysis. The pilA gene is 429bp long and it encodes protein associated with type Ⅳ fimbriae. The data implies that the pili on the surface of X. campestris pv. vesicatoria 833 interact with bacteriocin glyR, and as the result the growth of X. campestris pv. vesicatoria 833 is inhibited. For further studies on the protein interaction between the glyR and the PilA as the receptor, the wild type pilA gene was cloned.