AJOU Central Library Repository: GDF15 derived from Senescent Fibroblasts Stimulates Melanogenesis in Human Melanocytes

Graduate School of Ajou University Department of Biomedical Sciences 3. Theses(Master)

GDF15 derived from Senescent Fibroblasts Stimulates Melanogenesis in Human Melanocytes

Alternative Abstract: Background Senescent fibroblasts play a role in aging pigmentation. In our previous RNA sequencing data in senescent fibroblasts, the Growth differentiation factor 15 (GDF-15) gene was found to be significantly upregulated. Objective We investigated the role of senescent fibroblast-derived GDF15 in the regulation of human pigmentation. Methods Normal human melanocytes were co-cultured with fibroblasts infected with GDF15 lenti-virus or sh-GDF15 and melanogenesis was analyzed. The pigmentation were also assessed in the ex vivo skin culture. Results The melanin contents and tyrosinase activity were significantly increased in melanocytes co-cultured with fibroblasts infected with GDF15 lenti-virus. The mRNA and protein expression levels of melanogenesis-associated proteins, microphthalmia-associated transcription factor (MITF) and tyrosinase were significantly up-regulated. The GDF15 stimulated b-catenin signaling in melanocytes. Consistently, the pigmentation were significantly reduced in melanocytes co-cultured with fibroblasts infected with sh-GDF15. The stimulatory action of GDF15 in pigmentation was further confirmed in ex vivo cultured skin. Conclusion Senescent fibroblast-derived GDF15 stimulates skin pigmentation and it may play a role in the aging pigmentation.

Appears in Collections:: Graduate School of Ajou University > Department of Biomedical Sciences > 3. Theses(Master)

qrcode

AJOU Central Library Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.