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GDF15 derived from Senescent Fibroblasts Stimulates Melanogenesis in Human Melanocytes
- Alternative Title
- Yeongeun Kim
- Author(s)
- 김영은
- Alternative Author(s)
- Yeongeun Kim
- Advisor
- 강희영
- Department
- 일반대학원 의생명과학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2019--8
- Language
- eng
- Alternative Abstract
- Background Senescent fibroblasts play a role in aging pigmentation. In our previous RNA sequencing data in senescent fibroblasts, the Growth differentiation factor 15 (GDF-15) gene was found to be significantly upregulated. Objective We investigated the role of senescent fibroblast-derived GDF15 in the regulation of human pigmentation. Methods Normal human melanocytes were co-cultured with fibroblasts infected with GDF15 lenti-virus or sh-GDF15 and melanogenesis was analyzed. The pigmentation were also assessed in the ex vivo skin culture. Results The melanin contents and tyrosinase activity were significantly increased in melanocytes co-cultured with fibroblasts infected with GDF15 lenti-virus. The mRNA and protein expression levels of melanogenesis-associated proteins, microphthalmia-associated transcription factor (MITF) and tyrosinase were significantly up-regulated. The GDF15 stimulated b-catenin signaling in melanocytes. Consistently, the pigmentation were significantly reduced in melanocytes co-cultured with fibroblasts infected with sh-GDF15. The stimulatory action of GDF15 in pigmentation was further confirmed in ex vivo cultured skin. Conclusion Senescent fibroblast-derived GDF15 stimulates skin pigmentation and it may play a role in the aging pigmentation.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Biomedical Sciences > 3. Theses(Master)
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