Biosensing based on selective light absorption and wavelength filtering from enzymatic chromogenic reaction

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dc.description학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2019. 8-
dc.description.tableofcontents1. Introduction 1 1.1 Optical analysis in point-of-care testing 1 1.2 Smartphone-based POCT devices 2 1.3 Count-based analysis using selective light absorption and wavelength filtering 3 1.4 Aim of thesis 7 2. Materials and methods 8 2.1 Reagents and apparatus 8 2.2 Preparation of the paper-based signal guide 8 2.3 Fabrication of the reaction channel 9 2.3.1 Fabrication of the transparent biosensing channel and surface modification using polydopamine coating 9 2.3.2 Formation of the biorecognition layer for competitive immunoassay 10 2.4 uCTX-II competitive immunoassay 12 2.5 Preparation of the smartphone gadget 12 3. Results and discussion 14 3.1 Signaling principle of the count-based optical analysis 14 3.1.1 Inducing the chromogenic reaction for specific wavelength filtering effect 14 3.1.2 Principle of the count-based analysis for uCTX-II using signal guide 16 3.2 Verification of the signaling principle by filtering effect 19 3.3 Optimized results for maximum color development 25 3.3.1 Optimization the biosensing surface conditions and TMB reaction time 25 3.3.2 Optimization the ratio of antigen to antibody 29 3.4 Measurement of uCTX-II 32 3.4.1. Smartphone gadget for count-based analysis 35 3.4.2. Count-based uCTX-II analysis using smartphone 37 4. Conclusions 41 5. References 42-
dc.publisherThe Graduate School, Ajou University-
dc.rights아주대학교 논문은 저작권에 의해 보호받습니다.-
dc.titleBiosensing based on selective light absorption and wavelength filtering from enzymatic chromogenic reaction-
dc.title.alternativeSaemi Kim-
dc.contributor.affiliation아주대학교 일반대학원-
dc.contributor.alternativeNameSaemi Kim-
dc.contributor.department일반대학원 분자과학기술학과- 8-
dc.description.alternativeAbstractWe developed a simple optical biosensing system using the count-based analysis method based on the selective light absorption and the specific wavelength filtering effect. The developed system used a gadget that consisted of a paper-based signal guide, a biosensing channel, and a smartphone. The paper-based signal guide was prepared by printing red patterns of various brightness against a dark background. Since a blue product was generated by the horseradish peroxidase (HRP)-based enzymatic reaction in the biosensing channel, the channel was used as a blue filter that can absorb the red light. When the red light reflected from the red pattern was absorbed by the channel, the pattern was observed as black. In other words, since the color of the patterns was assimilated with the background, the pattern seemed to disappear. Consequently, the amount of the blue product related to the concentration of target analyte can be measured by counting the number of observed patterns on the paper-based signal guide. In this study, the concentration of urinary C-telopeptide fragment of type II collagen (uCTX-II, 0-10 ng/mL) was measured using the developed system without complicated equipment. Therefore, we expect that the developed optical transducing system could be applied for point-of-care testing (POCT).-
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Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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