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건선에서 자가조립 히알루론산 나노입자의 기능 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김욱 | - |
| dc.contributor.author | 이은영 | - |
| dc.date.accessioned | 2019-04-01T16:41:31Z | - |
| dc.date.available | 2019-04-01T16:41:31Z | - |
| dc.date.issued | 2019--2 | - |
| dc.identifier.other | 28610 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/15094 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2019. 2 | - |
| dc.description.tableofcontents | Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS and METHODS 5 1. Materials 5 2. Preparation and characterization of HA-LCA NPs 6 3. Mice and induction of psoriasis mouse model 7 4. Fluorescence microscopy for the transdermal delivery of HA-LCA NPs 8 5. Histological analysis 9 6. Cells and treatments 9 7. Cell viability test 10 8. RNA extraction and real-time reverse transcription polymerase chain reaction (RT-qPCR) 11 9. Statistical analysis 11 Ⅲ. RESULTS 13 1. Transdermal penetration of HA-LCA NPs in mouse skin 13 2. HA-LCA NPs treatment alleviates psoriatic features in IMQ-induced mouse psoriasis model 18 3. HA-LCA NPs reduces keratinocyte differentiation and immune infiltrates 22 4. Inhibitory effects of HA-LCA NPs on mRNA expression of proinflammatory cytokines in keratinocyte and macrophage cell lines 25 Ⅳ. DISCUSSION 31 Ⅴ. REFERENCE 34 Ⅵ. ABSTRACT IN KOREAN (국문초록) 39 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 건선에서 자가조립 히알루론산 나노입자의 기능 연구 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2019. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 905334 | - |
| dc.identifier.uci | I804:41038-000000028610 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000028610 | - |
| dc.description.alternativeAbstract | Since hyaluronic acid (HA) has good biocompatibility and nonimmunogenicity, self-assembled hyaluronic acid nanoparticles (HA-NPs) have been widely used for a drug carrier combined with active agents. Recently, it has been reported that empty HA-NPs not bearing any drugs have therapeutic effects on adipose tissue inflammation and insulin resistance. In this study, I investigated effects of HA-NPs itself on psoriasis; chronic and relapsing inflammatory skin disease. HA-NPs is an amphiphilic molecule that is composed with hydrophobic lithocholic acid (LCA) and hydrophilic HA, so it is self-assembled in particles which have hydrophilic surface and hydrophobic core in aqueous environment. In the imiquimod (IMQ)-induced mouse psoriasis model, topical treatment with HA-LCA nanoparticles (HA-LCA NPs) effectively reduced IMQ-induced epidermal proliferation, abnormal differentiation, and macrophage infiltration. Using monocyte cell line, I found that HA-LCA NPs inhibit monocyte differentiation into pro-inflammatory M1 macrophage and reduce expression levels of tumor necrosis factor-alpha (TNF-α), which is the central player of inflammation and psoriasis and induces inflammatory cascades and hyper-proliferation of keratinocyte. In addition, HA-LCA NPs also down-regulated TNF-α and interferon-gamma-induced expression of pro-inflammatory cytokine genes in keratinocyte cell line. Thus, the skin-permeating HA-LCA NPs may serve as a new therapeutic agent for psoriasis treatment. | - |
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- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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