repository
MARCH5에 의한 RIG-I/MAVS 신호전달의 조절
- Alternative Title
- Seong-Gwang Kim
- Author(s)
- 김성광
- Alternative Author(s)
- Seong-Gwang Kim
- Advisor
- 조혜성
- Department
- 일반대학원 의생명과학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2019-02
- Language
- eng
- Alternative Abstract
- The innate immune system has an important role in the mammalian immune response. Recent study has demonstrated that mitochondria take part in a broad range of innate immune pathways, functioning as signal platforms and resulting in activation of immune responses. Previously, our lab found that MARCH5 regulates immune response by resolving MAVS prion-like aggregates during viral infection. The present study revealed the kinetics of RIG-I/MAVS oligomerization upon poly(I:C) transfection. RIG-I and MAVS began to form oligomer, respectively after 12 hrs of poly(I:C) transfection. This was accompanied with accumulation of RIG-I and MAVS protein levels. At 36hrs after stimulation, the oligomerized RIG-I and MAVS proteins were accumulated in the NP_40-insoluble fractions, suggesting that the RIG-I/MAVS oligomers form a strong insoluble protein aggregates in cells. I found that MARCH5 not only targeted the oligomerized MAVS protein but also caused a significant reduction of the oligomerized RIG-I levels, of which degradation is mediated through a proteasome-dependent manner. In bone-marrow derived macrophage cell (BMDM) from MARCH5 knockout mice secreted high amount of type-I interferon secretion in response to poly(I:C) transfection. Approach to oligomeric complex provides understanding of relationship between RIG-I/MAVS oligomers and MARCH5. That make the reason I proposed that RIG-I and MAVS oligomers can be dual-targeted by MARCH5 during innate immune response.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Biomedical Sciences > 3. Theses(Master)
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
-
- License
- STATISTICS
- Total Visit :2,955,753
- Total Download :1,694
- Today View :2,139
