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초음파 활성 나노 입자를 이용한 탈모 치료를 위한 Cas9-RNP 전달 시스템
- Alternative Title
- Cas9-RNP delivery system for hair loss therapy by an ultrasound active particels
- Author(s)
- 이한아름
- Alternative Author(s)
- Lee Han A Reum
- Advisor
- 윤태종
- Department
- 일반대학원 약학
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2017-08
- Language
- eng
- Keyword
- ultrasound; microbubble; nanoliposome; CRISPR/Cas9; androgenic alopecia
- Alternative Abstract
- Protein-based therapy has conventional limitations because of its low efficiency and the difficulty of targeted delivery. To overcome this, we designed that lecithin-based liposome (NL) containing a Cas9/sgRNA ribonucleoprotein (Cas9-RNP) complex were linked to a microbubble (MB). Importantly, the bursting of the MB under acoustic pressure released the NL and enhanced their intracellular delivery owing to a sonoporation effect. We demonstrated that the Cas9-RNP within the NL used for SRD5A2 gene editing improved cell proliferation by blocking conversion of testosterone from dihydrotestosterone (DHT) which induces cell death. Interestingly, multiple treatments of the microbubble-nanoliposome (MN) exhibited higher efficacy than a single treatment. In summary, these results demonstrated that the ultrasound active MN system incorporating Cas9-RNP for hair loss therapy enhanced gene editing efficiency in human dermal papilla cells by sonoporation. Also, we expect that this MN system reduce side effects by provide opportunities to local delivery of Cas9-RNP to dermal papilla cells in hair follicles.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Pharmacy > 3. Theses(Master)
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