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Tissue regeneration study using three-dimensional scaffold fabricated by 3D printing technology
- Alternative Title
- Tissue regeneration study using three-dimensional scaffold fabricated by 3D printing technology
- Author(s)
- Kwon, Doo Yeon
- Alternative Author(s)
- Doo Yeon Kwon
- Advisor
- 김문석
- Department
- 일반대학원 분자과학기술학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2016-02
- Language
- eng
- Keyword
- Tissue regeneration; 3D scaffold; 3D printing
- Alternative Abstract
- Tissue engineering has significant potential to restore the damaged bone. Bone tissue engineering, including the scaffold, cells, and growth factors as a component, can overcome the drawbacks of conventional treatment methods which comes from lowering of curative ability and pathogenic infections. Manufacturing of scaffolds using 3D bioprinting for bone tissue engineering could be a useful technology in the treatment of bone defects and a variety of studies have been conducted. Biomaterials used in 3D bioprinting must have a biocompatibility and suitable biodegradability to apply in bone tissue engineering. The 3D bioprinted scaffold must have a suitable in vivo degradation period as well as provide a good environment for cells attachment and growth. Additionally, it must fully degrade in human body with inducing osteogenesis. In this study, biodegradable polyesters (bio-inks) having various degradation periods were synthesized to apply in 3D bioprinting. Also, the scaffolds for bone tissue engineering were fabricated by using 3D bioprinting. In addition, the 3D bioprinted scaffolds with the cells and bone growth factors were implanted in bone defect site for evaluation of new bone formation. In chapter 1, the background about tissue engineering, 3D bioprinting technology, and the application of 3D bioprinted scaffolds has been described. Chapter 2 demonstrates the preparation and feasibility evaluation of three-dimensional scaffolds by using 3D bioprinting. To apply the material for 3D bioprinting, various bio-inks were designed and synthesized by ring-opening polymerization. The bio-inks composed of ε-caprolactone (CL), L-lactide (LA), and glycolide (GA) were prepared with a variety of molecular weight and monomer ratio. The obtained bio-inks were applied in 3D bioprinting to evaluate the printability as the materials for 3D bioprinting. It was confirmed that the 3D bioprinted scaffolds have a well-defined and porous structure. In addition, the degradation rate was controlled depending on the kind of bio-inks showing excellent biocompatibility. In Chapter 3, poly (PLLA-co-PGA-co-PCL) (PLGC) scaffold fabricated by 3D bioprinting and human dental pulp stem cells (hDPSCs) were used to generate neo-bone formation in bone defect. The hDPSCs showed high proliferation rate and osteogenic differentiation in presence of osteogenic factors with bone morphogenetic protein-2 (rhBMP-2). The PLGC scaffold was implanted into cranial bone defect with hDPSCs and rhBMP-2. Neo-bone formation was confirmed by micro-computed tomography (CT) and histology. Also, the PLGC scaffold was degraded gradually and it showed good correlation between scaffold degradation and new bone formation. In conclusion, the results of this study show an ideal platform of bone regeneration using the scaffold fabricated by 3D bioprinting for treatment of bone defects.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 4. Theses(Ph.D)
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