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엔테로코커스 페컬리스 유래의 독성인자의 동정과 응용: 특성, 고정화, 하이드로젤 형성
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김두헌 | - |
| dc.contributor.author | 장은진 | - |
| dc.date.accessioned | 2018-11-08T08:17:56Z | - |
| dc.date.available | 2018-11-08T08:17:56Z | - |
| dc.date.issued | 2014-02 | - |
| dc.identifier.other | 16376 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/12545 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2014. 2 | - |
| dc.description.abstract | The virulence factors from pathogenic bacteria are useful for industrial applications because of their high stabilities and catalytic properties under harsh conditions. Here, characterization, immobilization, and hydrogel formation of a virulence factor, namely, EfEstA from Enterococcus faecalis are presented. The molecular characteristics of EfEstA were investigated by performing SDS-PAGE, zymogram assay, dynamic light scattering, mass spectrometry, enzyme assays, molecular modeling, circular dichroism spectroscopy, and fluorescence analysis. In addition, cross-linked enzyme aggregates (CLEAs) of EfEstA exhibited high recycling processes and improved stabilities compared to free EfEstA. Furthermore, macroscopic hydrogels of EfEstA could be used as an environment friendly carrier to absorb several metal ions. These characteristics of EfEstA shed light on the design and fabrication of new biomaterials based on bacterial virulence factors for the pharmaceutical, biotechnological, and chemical industries. | - |
| dc.description.tableofcontents | Contents Abstract ⅰ List of Figures ⅱ Ⅰ. Introduction 1 Ⅱ. Materials and methods 3 2.1 Bacterial strains, enzymes, and reagents 3 2.2 Sequence analysis and homology modeling 3 2.3 Cloning, expression, and purification of EfEstA 4 2.4. Enzyme assays 4 2.5. Circular dichroism and fluorescence analysis 5 2.6. Activity and aggregation in high salt conditions 5 2.7. Preparation of crosslinked enzyme aggregates (CLEAs) 6 2.8. Hydrogel formation of EfEstA 6 Ⅲ. Results and Discussion 8 3.1 Bioinformatic analysis of EfEstA 8 3.2 Biochemical analysis of EfEstA 11 3.3 Structural analysis of EfEstA 16 3.4 Salt- and chemical-stabilities of EfEstA 19 3.5 CLEAs of EfEstA 25 3.6 Hydrogel formation of EfEstA 27 Ⅳ. Conclusions 29 Ⅴ. Appendix 47 5.1 Introduction 48 5.2 Materials and Methods 50 5.3 Results and discussion 53 5.4. Conclusions 63 Reference 64 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 엔테로코커스 페컬리스 유래의 독성인자의 동정과 응용: 특성, 고정화, 하이드로젤 형성 | - |
| dc.title.alternative | Eun-Jin Jang | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Eun-Jin Jang | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2014. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 608267 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000016376 | - |
| dc.subject.keyword | virulence factor | - |
| dc.subject.keyword | CLEAs | - |
| dc.subject.keyword | Hydrogel | - |
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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