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In Vivo Osteogenic Differentiation of Human Dental Pulp Stem Cell in an Injectable In Situ-Forming Hydrogel
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김문석 | - |
| dc.contributor.author | 장자용 | - |
| dc.date.accessioned | 2018-11-08T08:16:09Z | - |
| dc.date.available | 2018-11-08T08:16:09Z | - |
| dc.date.issued | 2016-02 | - |
| dc.identifier.other | 22027 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/12082 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :분자과학기술학과,2016. 2 | - |
| dc.description.tableofcontents | 1. Introduction 2 2. Experimental 5 3. Results 13 4. Discussion 30 5. Conclusion 34 6. Reference 35 Abstract (in Korean) 40 List of Publications 41 | - |
| dc.language.iso | kor | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | In Vivo Osteogenic Differentiation of Human Dental Pulp Stem Cell in an Injectable In Situ-Forming Hydrogel | - |
| dc.title.alternative | In Vivo Osteogenic Differentiation of Human Dental Pulp Stem Cell in an Injectable In Situ-Forming Hydrogel | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Ja Yong Jang | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2016. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 739420 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000022027 | - |
| dc.subject.keyword | stem cell | - |
| dc.subject.keyword | osteogenesis | - |
| dc.subject.keyword | hydrogel | - |
| dc.description.alternativeAbstract | In this study, human dental pulp stem cells (hDPSCs) were examined as a cellular source for bone tissue engineering using an in vivo-forming hydrogel. The hDPSCs are easily harvested in large quantities from extracted teeth that are discarded. The stemness of harvested hDPSCs indicated their relative tolerance to ex vivo manipulation in culture. The in vitro osteogenic differentiation of hDPSCs was characterized using Alizarin Red S (ARS), von Kossa (VK), and alkaline phosphatase (ALP) staining. The solution of hDPSCs and a methoxy polyethylene glycol-polycaprolactone block copolymer (MPEG-PCL (PC)) is easily prepared by simple mixing at room temperature and almost immediately it forms in vivo hydrogels after subcutaneous injection into rats. The hDPSCs embedded in the in vivo-forming PC hydrogel survived for more than at least 6 weeks and induced comparably little inflammation. In vivo osteogenic differentiation of hDPSCs in the in vivo-forming PC hydrogel was confirmed by micro-CT, histological staining, and gene expression. Micro-CT analysis showed evidence of significant tissue-engineered bone formation in hDPSCs-loaded PC hydrogel in the presence of osteogenic factors. Differentiated osteoblasts in in vivo-forming hydrogels were identified by ARS and VK staining and were found to exhibit characteristic expression of genes like osteonectin (ON), osteopontin (OP), and osteocalcin (OC) at different time points. In conclusion, hDPSCs embedded in an in vivo-forming PC hydrogel may provide benefits as a non-invasive formulation for bone tissue engineering applications. | - |
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- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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