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Development of the cell culture monitoring system using a smartphone-mountable paper-based analytical device
- Author(s)
- 임성현
- Advisor
- 윤현철
- Department
- 일반대학원 분자과학기술학과
- Publisher
- The Graduate School, Ajou University
- Publication Year
- 2017-02
- Language
- eng
- Keyword
- Biosensor
- Alternative Abstract
- We developed a biosensing system for the facile monitoring of animal cell culture using paper-based analytical device (PAD) and a smartphone. Because that the animal cells consume glucose and produce lactate under the anaerobic culturing condition, the PAD was designed to simultaneously quantify the concentrations of glucose and lactate in culture media for cell culture monitoring. As a biosensing strategy, the enzyme-mediated colorimetric assay method, utilizing oxidase, horseradish peroxidase (HRP) and chromogenic substrate, were employed. On the PAD, two types of enzyme pairs including glucose oxidase (GOx)-HRP pair and lactate oxidase (LOx)-HRP pair were immobilized with colorless chromophores. In this condition, by the cascadic enzyme reactions among target substrates, oxidases, HRP and chromophore, a concentration-dependent colorimetric (blue) signals is developed on the PAD. With a smartphone gadget designed to aid the image acquisition, images of the PAD were acquired using a mobile application, and the color intensities were quantified as sensor signals. For the glucose assay, the developed biosensing system exhibited 0.3 mM of detection limit (LOD) and 0.9 mM of quantification limit (LOQ) in the dynamic detection range from 0.3 to 8.0 mM of glucose. For lactate biosensing, the LOD and LOQ values were respectively registered as 0.02 mM and 0.06 mM in the dynamic detection range from 0.02 to 0.5 mM of lactate. With the developed biosensing system, simultaneous quantification of glucose and lactate in cell culture media were conducted, exhibiting highly accurate and reproducible results. Based on the results, we propose that the developed optical sensing system using paper-based fluidics is feasible for practical monitoring of animal cell culture.
- Fulltext
- Appears in Collections:
- Graduate School of Ajou University > Department of Molecular Science and Technology > 3. Theses(Master)
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