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Preparation and Characterization of Self-assembled Drug-Albumin Nanoparticles for Active Tumor Targeting
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이범진 | - |
| dc.contributor.author | 길명철 | - |
| dc.date.accessioned | 2018-11-08T07:59:28Z | - |
| dc.date.available | 2018-11-08T07:59:28Z | - |
| dc.date.issued | 2013-02 | - |
| dc.identifier.other | 13546 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/9489 | - |
| dc.description | 학위논문(석사)아주대학교 일반대학원 :약학,2013. 2 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Preparation and Characterization of Self-assembled Drug-Albumin Nanoparticles for Active Tumor Targeting | - |
| dc.title.alternative | GIL Myung-Chul | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | GIL Myung-Chul | - |
| dc.contributor.department | 일반대학원 약학 | - |
| dc.date.awarded | 2013. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 570843 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000013546 | - |
| dc.subject.keyword | Irinotecan | - |
| dc.subject.keyword | Bovine serum albumin (BSA) | - |
| dc.subject.keyword | Drug-BSA conjugation | - |
| dc.subject.keyword | IRT-PMPI-BSA nanoparticles | - |
| dc.subject.keyword | Active targeting | - |
| dc.subject.keyword | Self-assembly | - |
| dc.description.alternativeAbstract | The aim of this study was to prepare anticancer drug-bovine serum albumin (BSA) conjugates using a cross-linker to prepare self-assembling nanoparticles. Irinotecan (IRT) was chosen as a model anticancer drug. N-[p-maleimidophenyl]-isocyanate (PMPI) was chosen as the cross-linker. An IRT-PMPI intermediate containing a maleimide moiety was reacted with the BSA sulfhydryl groups in solution. Residual non-reacted IRT was removed using micro-scale desalting column chromatography. After freeze-drying, the IRT-PMPI-BSA nanoparticles, in the form of a yellow powder, were obtained. The physicochemical properties of the IRT-PMPI-BSA nanoparticles were then investigated using Fourier transform infrared spectroscopy (FT-IR) analysis, dynamic light scattering (DLS), powder X-ray diffraction (PXRD) and transmission electron microscopy (TEM). The critical aggregation concentration (CAC), loading content and encapsulation efficiency of IRT-loaded IRT-PMPI-BSA nanoparticles were measured. FT-IR, PXRD and CAC data confirmed that IRT-PMPI-BSA nanoparticles had been synthesized successfully. TEM and DLS data showed that the nanoparticles formed due to self-assembly of the hydrophobic drug and hydrophilic BSA and that the nanoparticles were spherical and on the nanometer scale. The loading content and encapsulation efficiency of IRT, determined using HPLC, varied with reaction conditions. These IRT-conjugated BSA nanoparticles may be used for targeting of active tumors. | - |
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- Graduate School of Ajou University > Department of Pharmacy > 3. Theses(Master)
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