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신경교세포에서 Matrix Metalloproteinase-3의 전사 조절에 대한 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 주일로 | - |
| dc.contributor.author | 김광수 | - |
| dc.date.accessioned | 2022-11-29T03:01:21Z | - |
| dc.date.available | 2022-11-29T03:01:21Z | - |
| dc.date.issued | 2006-02 | - |
| dc.identifier.other | 1328 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/21059 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :의학과,2006. 2 | - |
| dc.description.tableofcontents | ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES v LIST OF TABLES vi ABBREVIATION vii I. INTRODUCTION 1 II. MATERIAL AND METHOD 9 A. Reagents 9 B. Cell cultures 9 C. Reverse Transcription (RT)- PCR Analysis 9 D. Western Blot Analysis 10 E. Electrophoretic Mobility Shift Assay (EMSA) 11 F. RAT MMP-3 Promoter-Reporter Constructs 13 G. Transfection 14 F. Site-Direct Mutagenesis 14 III. RESULT 16 A. Induction of MMP-3 mRNA and protein expression in rat primary astrocyte. 16 B. Distal and middle AP-1 of MMP-3 promoter confers transcriptional regulation of MMP-3 in rat astrocytes. 19 C. Stimuli activation of AP-1 binding function activates MMP-3 transcript. 24 D. Both distal- and middle-AP-1 element were important on expression of MM-3 gene. 28 E. Activation up-stream of AP-1 signal pathways and MMP-3 expression only repressed by JNK inhibitor. 31 F. MMP-3 expression and MMP-3 5…-Flanking promoter function were interfered with LXR agonists. 35 G. LXR agonists inhibited AP-1 element in MMP-3 promoter region. 39 IV. DISCUSSION 42 V. CONCLUSION 46 REFERENCES 49 국문요약 58 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 신경교세포에서 Matrix Metalloproteinase-3의 전사 조절에 대한 연구 | - |
| dc.title.alternative | Transcriptional Regulation of Matrix MetalloProteinase-3 in Rat Brain Astrocytes. | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | kim kwang soo | - |
| dc.contributor.department | 일반대학원 의학과 | - |
| dc.date.awarded | 2006. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 565068 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000001328 | - |
| dc.description.alternativeAbstract | Matrix metalloproteinases (MMPs) are zinc-dependent endopeptidases that degrade extracellular matix (ECM) components during normal and pathogenic condition. Documented expression of MMPs contributes to tissue remodeling, atherosclerosis, and inflammatory diseases. MMPs are tight regulated by several steps including transcriptional regulation. Transcription factors, NF-？？B, Ets and activator protein (AP)-1 are reported to be involved in transcriptional regulation of MMPs. Here we demonstrated transcriptional regulations of MMP-3 in LPS-, IFN？^-, and gangliosides-activated cultured astrocytes obtained from rat brain. MMP-3 transcript and protein were increased by stimulation with LPS, IFN？^ and gangliosides. Promoter analysis revealed that sequences between -500 to start codon are important in induction of MMP-3. EMSA-based study and site-direct mutagenesis revealed that AP-1 is important in induction of MMP-3. Since three AP-1 binding sites (we described them as proximal, middle and distal, respectively according to the distances from start codon) exist in -500 promoter sites, we tested which AP-1 site is important using sequence-specific primers for EMSA. We observed that middle and distal AP-1, not proximal AP-1 are activated by all three stimulators in EMSA analysis. Further, we demonstrated that LXR activation inhibit MMP-3 transcription through interaction with AP-1. | - |
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- Graduate School of Ajou University > Department of Medicine > 3. Theses(Master)
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