repository
Effect of brain slice conditioned media (SCM) on differentiation of monocytes into microglia.
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 박상면, 조은혜 | - |
| dc.contributor.author | 고동완 | - |
| dc.date.accessioned | 2022-11-29T03:01:21Z | - |
| dc.date.available | 2022-11-29T03:01:21Z | - |
| dc.date.issued | 2022-02 | - |
| dc.identifier.other | 31618 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/21048 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :의생명과학과,2022. 2 | - |
| dc.description.tableofcontents | Ⅰ. INTRODUCTION 1 A. Microglia. 1 B. Monocytes. 1 C. Monocytes in the damaged brain. 2 D. Infiltrating monocytes differentiated into microglia . 2 E. Specific Aims. 3 Ⅱ. MATERIALS AND METHODS. 4 A. Animal. 4 B. Stereotaxic injection 4 C. Immunostaining. 4 D. Monocytes culture. 5 E. Slice culture, preparation of slice culture conditioned media. 5 F. Western blot. 6 G. Statistical analysis. 6 Ⅲ. RESULTS 8 A. CD45(+) monocytes expressed Tmem119 in ATP injected brain. . 8 B. LPS did not induce differentiation of monocytes into microglia. . 10 C. Brain slice culture-conditioned media (SCM) induced differentiation of monocytes into microglia. 12 D. Immunocytochemistry: Expression of Tmem119 in monocytes induced by SCM. 14 E. SCM enhanced phagocytic activity in monocytes. 15 F. None of ACM, NCM, ATP, glutamate, and Conditioned media from ATP- or glutamate-treated astrocytes and neuron induced differentiation of monocytes into microglia. 16 Ⅳ. DISCUSSION 17 REFERENCE 19 국문요약. 25 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Effect of brain slice conditioned media (SCM) on differentiation of monocytes into microglia. | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2022. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 1245064 | - |
| dc.identifier.uci | I804:41038-000000031618 | - |
| dc.identifier.url | https://dcoll.ajou.ac.kr/dcollection/common/orgView/000000031618 | - |
| dc.subject.keyword | Microglia | - |
| dc.subject.keyword | Monocytes | - |
| dc.subject.keyword | Slice conditioned media | - |
| dc.subject.keyword | Tmem119 | - |
| dc.description.alternativeAbstract | It has been reported that monocytes entered the injured brain, and differentiate into microglia, resident brain macrophages. In this study, I found that slice culture conditioned media (SCM) induced expression of Tmem119, a microglia specific marker, in bone marrow-derived monocytes (BMDM). In addition, SCM also increased phagocytic activity of monocytes. However, Tmem119 expression was not changed in the presence of a TLR4 agonist (LPS), or culture conditioned media from astrocytes (ACM) and neurons (NCM) treated with or without insults such as glutamate and ATP. These results suggest that factors from several types of cells in damaged brain tissues may cooperate to induce differentiation of monocytes into microglia. | - |
- Appears in Collections:
- Graduate School of Ajou University > Department of Biomedical Sciences > 3. Theses(Master)
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
-
- License
- STATISTICS
- Total Visit :3,728,526
- Total Download :1,818
- Today View :6,185
