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Injectable Gelatin Hydrogels for Tissue Adhesive and Tissue Engineering
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 박기동 | - |
| dc.contributor.author | 류승배 | - |
| dc.date.accessioned | 2022-11-29T02:33:06Z | - |
| dc.date.available | 2022-11-29T02:33:06Z | - |
| dc.date.issued | 2022-02 | - |
| dc.identifier.other | 31758 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/20584 | - |
| dc.description | 학위논문(박사)--아주대학교 일반대학원 :분자과학기술학과,2022. 2 | - |
| dc.description.tableofcontents | Chapter I. General introduction 1 1. Biomaterials 2 1.1. Polymeric biomaterials 3 1.2. Gelatin as a biopolymer 6 1.3. Hydrogels 6 1.3.1. Cross-linking methods for injectable hydrogels 7 1.3.2. Enzyme-mediated cross-linking 10 2. Tissue adhesive and cardiac regeneration 13 2.1. Tissue adhesive 13 2.1.1. Mechanism of tissue adhesive 14 2.1.2 Requirements of adhesive in biomedical applications 16 2.1.3 Current adhesives and limitations 17 2.2. Cardiac tissue regeneration 22 2.2.1. Electroconductive hydrogels for cardiac regeneration 24 2.2.2. ROS consuming hydrogels for cardiac regeneration 27 2.2.3 Current hydrogels and limitations 28 3. Overall objective 31 4. References 32 Chapter 2. Graphene oxide incorporated gelatin hydrogels as an injectable tissue adhesive with robust mechanical and adhesive properties 36 1. Introduction 37 2. Materials and Methods 39 2.1. Materials 39 2.2. Synthesis of gelatin-hydroxyphenyl propionic acid (GH) 39 2.3. Fabrication of oxidized-graphene (GO) 40 2.4. Preparation of in situ forming GH hydrogels incorporating GO (GH/GO) 41 2.5. Measurement of gelation time 41 2.6. Tensile strength and tissue adhesive strength of the hydrogels 41 2.7. Proteolytic degradation study 42 2.8. In vitro cytotoxicity test 43 2.9. Statistical analysis 43 3. Results and discussion 44 3.1. Characterization of GH conjugate and GO 44 3.2. Preparation of in situ forming GH/GO hydrogels and gelation time 45 3.3. Tensile strength of the hydrogels 48 3.4. Tissue adhesive strength of hydrogels 50 3.5. Proteolytic degradation study 52 3.6. In vitro cytocompatibility 53 4. Conclusions 54 5. References 55 Chapter 3. Electroconductive ROS consuming gelatin/fucoidan hybrid hydrogels for cardiac tissue regeneration 57 1. Introduction 58 2. Materials and Methods 60 2.1. Materials 60 2.2. Synthesis of gelatin-hydroxyphenyl propionic acid (GH) and Fucoidan- tyramine (FTA) 60 2.3. Preparation of in situ forming GH/FTA hybrid hydrogels 61 2.4. Characterization of hydrogels 62 2.5. Electroconductivity test of hydrogels 62 2.6. ROS consuming behavior of hydrogels 63 2.7. Proteolytic degradation study 64 2.8. In vitro cytotoxicity and proliferation test 65 3. Results and discussion 65 3.1. Characterization of GH and F-TA polymers 65 3.2. Preparation of in situ forming GH/FTA hybrid hydrogels and gelation time 67 3.3. Elastic modulus of GH/FTA hybrid hydrogels 69 3.4. Fe ion induced electroconductivity of hydrogels 70 3.5. ROS consuming behavior of hydrogels 71 3.6. Proteolytic degradation behavior 73 3.7. In vitro cytocompatibility 74 4. Conclusions 76 5. References 77 Chapter 4. Overall conclusions 79 1. Overall conclusions 80 IV. Abstract in Korean 81 | - |
| dc.language.iso | kor | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Injectable Gelatin Hydrogels for Tissue Adhesive and Tissue Engineering | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Seung Bae Ryu | - |
| dc.contributor.department | 일반대학원 분자과학기술학과 | - |
| dc.date.awarded | 2022. 2 | - |
| dc.description.degree | Doctoral | - |
| dc.identifier.localId | T000000031758 | - |
| dc.identifier.uci | I804:41038-000000031758 | - |
| dc.identifier.url | https://dcoll.ajou.ac.kr/dcollection/common/orgView/000000031758 | - |
| dc.subject.keyword | Biomaterials | - |
| dc.subject.keyword | Hydrogel | - |
| dc.subject.keyword | Tissue Engineering | - |
| dc.description.alternativeAbstract | Hydrogels is hydrophilic polymeric three-dimensional (3D) networks structures that can absorb surrounding water or biological fluids. It is cross-linked via covalent bond or physical (intramolecular or intermolecular) interactions, Due to the extracellular matrix (ECM) mimicking structure of the hydrogel that considered as a promising artificial scaffold for many biomedical applications. Their 3D microstructure provides delivery of bioactive molecules such as drugs, cells, growth factors and release as sustained manner. The hydrogel matrix should provide proper spaces for the cells to migrate and proliferate, finally, replaced by ECM components that naturally secreted as the tissue regeneration process. Therefore, designing criteria by the application specific condition of hydrogels as tissue engineering scaffolds should be considered with the physical/chemical (mechanical strength, material-tissue interaction and degradation rate) and biological (cellular responses, modulate cellular behavior) requirements. Among these hydrogel systems, an enzyme-catalyzed cross-linking mechanism has been widely studied to alter currently used cross-linking systems. Horseradish peroxidase (HRP)-catalyzed reaction system enable to control of physical and chemical properties. The porphyrin HRP structure containing iron that inducing the coupling of phenol or aniline derivatives in the presence H2O2 as the oxidant. It was demonstrated that HRP-catalyzed cross-linking system could control of cross-linking rate and degree, both of which affect key hydrogel properties, such as gelation time, mechanical stiffness, swelling properties and degradation rate. In Chapter 2, injectable tissue adhesive hydrogels have been widely utilized to replace the use of surgical sutures and staples because of their ability to fill irregular defect and to interact with surrounding tissues during hydrogel formation. However, the insufficient tissue adhesive strength of these iv hydrogels still remaining as a challenge for achieving a stable wound closure. In this study, we developed enzyme-mediated crosslinking gelatin-hydroxyphenyl propionic acid (GH)/graphene oxide (GO) composite hydrogels to improve mechanical properties that can serve as an injectable tissue adhesive. The effect of different concentration of GO on the physico-chemical properties of GH hydrogels were investigated, particularly tissue adhesion strength. In vitro proteolytic degradation behavior and cyto-toxicity study confirmed the hydrogels were biodegradable and have an excellent cytocompatibility. In chapter 3, gelatin-hydroxyphenyl propionic acid (GH)/fucoidan-tyramine (FTA) hybrid with ROS consuming behavior and electroconductive was developed. The he physicochemical properties of GH/FTA hydrogels were investigated, particularly the ROS consumption behavior and electroconductivity of the hydrogels. We demonstrate that injectable GH/FTA hydrogels have potential to be used as a cardiac tissue regeneration platform | - |
| dc.title.subtitle | Effect of Graphene Oxide on Mechanical Properties and Fucoidan/Fe+3 on Electroconductivity and ROS Consumption | - |
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