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Senolytic Drugs are Potential Skin Lightening Agent for Aging Pigmentation
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 박태준 | - |
| dc.contributor.author | 박지희 | - |
| dc.date.accessioned | 2022-11-29T02:32:31Z | - |
| dc.date.available | 2022-11-29T02:32:31Z | - |
| dc.date.issued | 2021-02 | - |
| dc.identifier.other | 30539 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/20068 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :의생명과학과,2021. 2 | - |
| dc.description.abstract | 피부의 노화 과정은 색소 체계를 변형시키는 중요한 외적 요인이다. 세포 노화가 근본적인 노화 메커니즘이다. 노화 섬유아세포들이 노화 관련 색소 침착 부위에 축적되고 노화 중에 간질 상피 상호 작용을 통해 멜라닌세포 분화를 변화시킨다. 세놀리틱스 약물은 노화성에서는 세포 사멸을 유발할 수 있지만 비노화성 세포에서는 그렇지 않다. 세놀리틱스 약물로 잘 알려진 ABT263과 ABT737은 노화성이 많은 조직에 축적되며 노화성 세포에 사멸을 유도할 수 있다. ABT263과 ABT737은 멜라노사이트에서 멜라닌 합성에 관여하지 않았지만, 노화 섬유아세포 사멸의 조절하면서 멜라노사이트의 색소 침착을 감소시켰다. 우리는 다양한 세놀리틱스 약물 중에 특히 ABT263과 ABT737이 노화 섬유아세포에서 사멸을 유도하고 피부 색소를 조절하는 것을 밝혀내었다. | - |
| dc.description.tableofcontents | I. INTRODUCTION 1 II. MATERIALS AND METHODS 2 1. Materials 2 2. Cell culture 2 3. In vitro model of senescent fibroblasts 2 4. Cell viability test 3 5. Apoptosis assay 3 6. Caspase 3/7 activity assay 3 7. Melanin content and tyrosinase activity assay 4 8. Real-time PCR analysis 4 9. Western blot analysis 5 10. Statistical analysis 5 III. RESULTS 6 1. Specificity of senolytic drugs for senescent fibroblasts of the skin 6 2. ABT263/ABT737 decreased skin pigmentation in vitro 12 3. The elimination of senescent fibroblasts induces skin lightening 20 IV. DISCUSSION & CONCLUSION 22 V. REFERENCES 23 국문요약 25 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Senolytic Drugs are Potential Skin Lightening Agent for Aging Pigmentation | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Ji Hee Park | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2021. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 1203420 | - |
| dc.identifier.uci | I804:41038-000000030539 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000030539 | - |
| dc.subject.keyword | Senolytic drug | - |
| dc.subject.keyword | aging | - |
| dc.subject.keyword | senescent fibroblasts | - |
| dc.subject.keyword | skin pigmentation | - |
| dc.description.alternativeAbstract | Cutaneous aging process is an important factor which modifies the pigmentary system. Given that cellular senescence is a fundamental aging mechanism, senescent fibroblasts accumulated at the dermis are involved in melanocyte differentiation via stromal-epithelial interactions during aging. Both ABT263 and ABT737, which are well known senolytic drugs, led apoptosis of senescent cells accumulated in tissues. Senolytic drugs induce apoptosis only senescent, but not normal cells. In this study, they did not affect melanin synthesis of melanocytes, however, melanocytes’ pigmentation was decreased via regulation of senescent fibroblasts apoptosis. Therefore, senolytic drug, especially ABT263 and ABT737, induced apoptosis in senescent cell and regulated the skin pigmentation in the elderly. As a result, we improved skin pigmentation. | - |
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