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Bidirectional transcriptome analysis of rat bone marrow-derived mesenchymal stem cells and activated microglia in an in vitro coculture system
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 이광 | - |
| dc.contributor.author | 이다연 | - |
| dc.date.accessioned | 2022-11-29T02:32:13Z | - |
| dc.date.available | 2022-11-29T02:32:13Z | - |
| dc.date.issued | 2020-08 | - |
| dc.identifier.other | 30178 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/19716 | - |
| dc.description | 학위논문(박사)--아주대학교 일반대학원 :의생명과학과,2020. 8 | - |
| dc.description.tableofcontents | 1. Introduction 1 2. Materials and methods 5 2.1. Isolation and maintenance of rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) 5 2.2. Rat microglia primary cultures 7 2.3. Coculture of LPS-stimulated or non-stimulated microglia and rBM-MSCs 9 2.4. Total RNA isolation and microarray analysis 9 2.5. Transcriptomic analysis using Ingenuity Pathway Analysis 10 2.6. Quantitative real-time PCR (qPCR) 14 2.7. Migration assay 17 2.8. Statistical analysis 17 3. Results 18 3.1. Cellular movement-related transcriptomic changes in rBM-MSCs cocultured with LPS-stimulated microglia 18 3.2. Functional prediction of transcriptomic networks in rBM-MSCs cocultured with LPS-stimulated microglia 23 3.3. Increased migratory activity in rBM-MSCs cocultured with LPS-stimulated microglia 28 3.4. Transcriptomic analysis in LPS-stimulated microglia cocultured with rBM-MSCs 30 3.5. Functional prediction of transcriptomic networks and reduced inflammatory response in LPS-stimulated microglia cocultured with rBM-MSCs 34 4. Discussion 41 CONCLUSION 44 REFERENCES 45 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Bidirectional transcriptome analysis of rat bone marrow-derived mesenchymal stem cells and activated microglia in an in vitro coculture system | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2020. 8 | - |
| dc.description.degree | Doctoral | - |
| dc.identifier.localId | 1151627 | - |
| dc.identifier.uci | I804:41038-000000030178 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/common/orgView/000000030178 | - |
| dc.subject.keyword | bone marrow-derived mesenchymal stem cells | - |
| dc.subject.keyword | inflammation | - |
| dc.subject.keyword | microglia | - |
| dc.subject.keyword | migration | - |
| dc.subject.keyword | transcriptome analysis | - |
| dc.description.alternativeAbstract | Microglia contribute to the pathogenesis of brain diseases by regulating of neuroinflammation. Thus, targeting of neuroinflammation triggered by activated microglia in brain diseases has become a promising curative strategy. Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been shown to have therapeutic effects, resulting from the regulation of inflammatory conditions in the brain. In this study, Gene expression pattern in rat BM-MSCs (rBM-MSCs) cocultured with lipopolysaccharide- (LPS-) stimulated primary rat microglia were investigated using microarray analysis and the functional relationships were evaluated through Ingenuity Pathway Analysis (IPA). The effects of rBM-MSC on LPS-stimulated microglia were also assessed using a reverse coculture system and the same transcriptomic analysis. In rBM-MSCs, 67 genes were differentially expressed, which were highly related with migration of cells, compared to control. The gene network was predicted using IPA and LPS-stimulated primary rat microglia increase the migration of rBM-MSCs was validated by experiments. Reversely, expression patterns of the transcriptome in LPS-stimulated primary rat microglia cocultured with rBM-MSCs were changed. Results showed that 64 genes were altered, which were highly related with inflammatory response, compared to absence of rBM-MSCs. In the same procedure with the aforementioned, the prediction of the gene network and experimental validation showed that rBM-MSCs decrease the inflammatory response of LPS-stimulated primary rat microglia. These indicate that LPS-stimulated microglia increase the migration of rBM-MSCs and that rBM-MSCs reduce the inflammatory activity in LPS-stimulated microglia. The results of this study show complex mechanisms underlying the interaction between rBM-MSCs and activated microglia and may be supportive for the advance of stem cell therapy for brain diseases. This study is based on a previously published report | - |
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- Graduate School of Ajou University > Department of Biomedical Sciences > 4. Theses(Ph.D)
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