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단순포진 바이러스로 유도한 베체트병 마우스모델에서 Poly I:C 에 의해 유도된 IL-15Rα의 항 염증 효과에 관한 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 손성향 | - |
| dc.contributor.author | Choi, Ju young | - |
| dc.date.accessioned | 2022-11-29T02:31:55Z | - |
| dc.date.available | 2022-11-29T02:31:55Z | - |
| dc.date.issued | 2012-02 | - |
| dc.identifier.other | 12284 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/19401 | - |
| dc.description | 학위논문(석사)아주대학교 일반대학원 :의생명학과,2012. 2 | - |
| dc.description.tableofcontents | TABLE OF CONTENTS ABSTRACT ⅰ TABLE OF CONTENTS Ⅲ LIST OF FIGURE ⅴ LIST OF TABLES ⅶ Ⅰ. INTRODUCTION 1 Ⅱ. MATERIALS AND METHODS 3 A. Animal experiments 3 B. BD symptoms in a mouse model 3 C. In vivo administration of Poly I:C 4 D. Flow cytometric analysis of cell surface staining 4 E. ELISA 5 F. Reverse transcription PCR (RT-PCR) 5 G. Statistical analysis 6 Ⅲ. RESULTS 7 A. The frequencies of IL-15Rα expressing cells in BD mice compared with BDN mice 9 B. Poly I:C up-regulates expression of IL-15Rα in PBMC of normal mice 12 C. Poly I:C ameliorates HSV-induced BD symptoms 16 D. Poly I:C up-regulates the frequencies of IL-15Rα but not IL-7Rα (CD127) in BD mice 18 E. Poly I:C induces CD8+CD44+ memory T cells in BD mice 20 F. Poly I:C up-regulate CD122(IL-15/2Rβ) and CD8+CD122+ T cells in BD mice 24 G. Poly I:C down-regulates IL-23 receptor and IL-17A in BD mice 28 Ⅳ. DISCUSSION 30 Ⅴ. CONCLUSION 34 REFERENCES 36 국문요약 45 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 단순포진 바이러스로 유도한 베체트병 마우스모델에서 Poly I:C 에 의해 유도된 IL-15Rα의 항 염증 효과에 관한 연구 | - |
| dc.title.alternative | Ju Young Choi | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | Ju Young Choi | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2012. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 570277 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000012284 | - |
| dc.subject.keyword | Behcet's disease | - |
| dc.subject.keyword | Herpes Simplex virus | - |
| dc.subject.keyword | IL-15Rα | - |
| dc.subject.keyword | Poly I:C | - |
| dc.subject.keyword | memory CD8+ T cells | - |
| dc.subject.keyword | mouse model | - |
| dc.description.alternativeAbstract | Behcet’s disease (BD) is a chronic, multi-systemic disorder that has arthritic, intestinal, mucocutaneous, ocular, vascular, and central nervous system affects. Interleukin-15 receptor alpha (IL-15Rα) forms stable complex with IL-15 on the cell surface of activated monocytes and mediates the proliferation of memory CD8+ T cells. Recent studies informed that Polyinosinic:polycytidylic acid (Poly I:C) is an immunostimulant which boosts the generation of memory T cells though induction of IL-15 and IL-15Rα. We found that the frequencies of IL-15Rα were significantly lower in HSV-induced BD mouse model than control mice. Therefore, this study was conducted to determine whether Poly I:C supplementation could induce memory cells through up-regulation of IL-15Rα, and reduce inflammation in HSV-induced BD symptoms. To evaluate whether the expression of IL-15Rα could be increased by poly I:C in vivo, Poly I:C was administered into normal and BD mice. Then, Poly I:C administered BD mice were subsequently observed the change of symptoms until 17 days after the last injection. The frequencies of IL-15Rα and memory CD8+ T cells were significantly up-regulated in Poly I:C injected mice. Poly I:C improved the symptoms and decreased severity score through down-regulation of pro-inflammatory cytokines and elevated regulatory and memory T cells. In conclusion, these results suggest that IL-15Rα is an important factor for the regulation of symptoms in HSV-induced BD mice. | - |
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