repository
Special Graduate Schools
Graduate School of Clinical Pharmacy and Pharmaceutics
Department of Clinical Pharmacy and Pharmaceutics
3. Theses(Master)
대장암에서 노화 종양세포 특성에 관한 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김수동 | - |
| dc.contributor.author | 황주원 | - |
| dc.date.accessioned | 2019-10-21T07:31:30Z | - |
| dc.date.available | 2019-10-21T07:31:30Z | - |
| dc.date.issued | 2018-02 | - |
| dc.identifier.other | 27675 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/19157 | - |
| dc.description | 학위논문(석사)--아주대학교 글로벌제약임상대학원 :글로벌제약임상약학과,2018. 2 | - |
| dc.description.tableofcontents | I.INTRODUCTION 1 II.MATERIAL AND METHOD 3 A.Cases and samples 3 B.Preparation of SA- β -Gal staining reagent 3 C.SA- β -Gal staining and counter staining 4 D.Grading of SA- β -Gal staining 4 E.Double Immunohistochemistry 5 F.Immunohistochemical grading of senescent tumor cells 5 G.KRAS NRAS mutation Testing 6 H.Primary cell culture 6 I.Statistics 7 III.Results 8 A.Characteristics of SA-β-Gal staining in the colorectal cancers 8 B.Grading of SA-β-Gal staining in the colorectal cancers 11 C.Correlation between SA-β-Gal staining and immunohistochemistry 15 D.Correlation between grades of SA-β-Gal staining and the sex and the age of patients 17 E.Grades of SA-β-Gal staining and extents of primary tumor(pT) 19 F.Comparison between grades of SA-β-Gal staining and the degree of spread to regional lymph nodes(pN) 22 G.Correlation between grades of SA-β-Gal staining and the tumor size of patients 24 H.Correlation between grades of SA-β-Gal staining and the oncogene (KRAS, NRAS) of cancer 26 I.Primary cell culture 29 IV.DISCUSSION 34 V.CONCLUSION 38 REFERENCES 39 국문요약 45 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 대장암에서 노화 종양세포 특성에 관한 연구 | - |
| dc.title.alternative | Study on the Characteristics of Senescent Tumor Cells in the Colorectal Cancers | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 글로벌제약임상대학원 | - |
| dc.contributor.department | 글로벌제약임상대학원 글로벌제약임상약학과 | - |
| dc.date.awarded | 2018. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 800569 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000027675 | - |
| dc.subject.keyword | Cellular senescence | - |
| dc.subject.keyword | Senescent tumor cell | - |
| dc.subject.keyword | Colorectal cancer | - |
| dc.description.alternativeAbstract | Cellular senescence is a condition in which cell proliferation is inhibited irreversibly. Cellular senescence can be induced by several factors such as telomere shortening, oxygen stress, UV, or oncogenic stimulation. Among them, oncogene-induced senescence is induced by oncogene overexpression. Oncogene-induced senescence was initially considered as a barrier of cancer progression. Recently, however, oncogene-induced senescence could be found in advanced cancer and may have a positive effect on cancer progression. In present study, we investigated characteristics of senescence cells in colorectal cancers. We examined colorectal cancer tissues and clinicopathological data from 51 patients who underwent colorectal cancer resection at Ajou University Hospital between 2016 and 2017. We performed SA-β-Gal staining and graded according to the degree of staining. The correlation between grade of SA-β-Gal staining and clinicopathological data was analyzed. We also conducted primary culture to investigate the role of tumor senescent cells. We found that three different staining pattern of senescent cells in colorectal cancer tissues. We obtained no statistical results in the correlation between senescent cells and patient’s data. Although there were no statistical significance between senescent tumor cells and parameters of colorectal cancers, we found senescent tumor cells were more frequently present in early stage of colorectal cancers and in small sized colorectal cancers (p=0.054, p=0.070). Our data suggested that the possibility of correlation between senescent tumor cells and pathogenesis of the colorectal cancers is present. | - |
- Appears in Collections:
- Special Graduate Schools > Graduate School of Clinical Pharmacy and Pharmaceutics > Department of Clinical Pharmacy and Pharmaceutics > 3. Theses(Master)
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
-
- License
- STATISTICS
- Total Visit :3,861,186
- Total Download :1,950
- Today View :6,483
