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Special Graduate Schools
Graduate School of Clinical Pharmacy and Pharmaceutics
Department of Clinical Pharmacy and Pharmaceutics
3. Theses(Master)
나노섬유를 이용한 마우스 일차 간세포의 배양조건 확립
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 김수동, 곽종영 | - |
| dc.contributor.author | 한신웅 | - |
| dc.date.accessioned | 2019-10-21T07:31:27Z | - |
| dc.date.available | 2019-10-21T07:31:27Z | - |
| dc.date.issued | 2018-02 | - |
| dc.identifier.other | 27472 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/19150 | - |
| dc.description | 학위논문(석사)--아주대학교 글로벌제약임상대학원 :글로벌제약임상약학과,2018. 2 | - |
| dc.description.tableofcontents | Ⅰ. INTRODUCTION 1 A. Hepatocyte 1 B. Co-culture of hepatocytes and nonparenchymal cells 1 C. Three-dimension nano-fiber scaffold 2 D. Aims 2 Ⅱ. MATERIALS AND METHODS 4 A. PCL & PVA nanofiber 4 B. Cell line 4 C. Hepatocyte isolation procedure 4 D. Hepatocyte culture 5 E. Hepatocytes and 3T3 culture in nanofiber 6 F. Immunocytochemistry 6 G. Live and dead cell staining 7 III. RESULTS 8 A. Morphology of mouse primary hepatocyte. 8 B. Albumin secretion of mouse primary hepatocytes. 10 C. Live and dead cell staining of mouse primary hepatocytes. 12 D. Live and dead cell staining of 3T3 cells cultured in PCL fiber. 14 E. Live and dead cell staining of mouse primary hepatocytes cultured in PVA fiber. 16 Ⅳ. DISCUSSION 18 Ⅴ. CONCLUSION 20 REFERENCES 21 -국문요약- 25 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 나노섬유를 이용한 마우스 일차 간세포의 배양조건 확립 | - |
| dc.title.alternative | Studies on the 3D culture of mouse primary hepatocyte using nanofiber scaffold | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 글로벌제약임상대학원 | - |
| dc.contributor.department | 글로벌제약임상대학원 글로벌제약임상약학과 | - |
| dc.date.awarded | 2018. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 800550 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000027472 | - |
| dc.subject.keyword | PCL | - |
| dc.subject.keyword | PVA | - |
| dc.subject.keyword | 삼차원 세포배양 | - |
| dc.subject.keyword | 마우스 일차 간세포 | - |
| dc.subject.keyword | C57BL/6 쥐 | - |
| dc.description.alternativeAbstract | Parenchyma cells take an important major role in human livers. Hepatocytes grow in the liver with a variety of non-parenchymal cells and carry out chemical interactions. Three - dimensional nanofibers, PVA and PCL, are known to be suitable for biological experiments due to their biocompatibility and biodegradability. In this study, 3T3 and mouse primary hepatocyte co-culture conditions were prepared using PVA and PCL nanofibers so that the cells could grow in a similar condition to the existing organs in the test tube. The experiment was confirmed. The primary hepatocytes, which were isolated from mice, were more attached to PVA fibers and survived for a long time when co - cultured as compared to those cultured alone. | - |
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