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Clusterin이 TGF-β 신호전달 기전을 통해 색소형성과정에 미치는 영향
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 강희영 | - |
| dc.contributor.author | 이지운 | - |
| dc.date.accessioned | 2019-10-21T07:28:43Z | - |
| dc.date.available | 2019-10-21T07:28:43Z | - |
| dc.date.issued | 2017-02 | - |
| dc.identifier.other | 24092 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/18924 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :의생명과학과,2017. 2 | - |
| dc.description.tableofcontents | I. INTRODUCTION 1 II. MATERIALS AND METHODS 3 A. Cell culture 3 B. Enzyme-linked immunosorbent assay (ELISA) 3 C. Biopsy collection and immunohistochemical analysis 3 D. Lentivirus production 4 E. Preparation of the Lenti-CLU and Lenti-shCLU infected fibroblast derived concentrated conditioned medium 4 F. Melanin content and tyrosinase activity assay 5 G. Semiquantitative real-time PCR and RT-PCR 5 H. Western blot analysis 6 I. Ex vivo skin organ culture and pigmentation assay in cultured skin 7 J. Preparation of TGF type II receptor dominant negative adenovirus 7 K. Statistical analysis 8 III. RESULTS 9 1. CLU expression in aged skin and UV exposed skin. 9 2. CLU expression in skin cells and UV irradiated fibroblast. 14 3. Fibroblast derived CLU inhibits melanogenesis. 20 4. CLU inhibits pigmentation via TGF-β signaling pathway. 35 IV. DISCUSSION 40 V. REFERENCES 50 국문요약 53 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Clusterin이 TGF-β 신호전달 기전을 통해 색소형성과정에 미치는 영향 | - |
| dc.title.alternative | Clusterin negatively regulates melanogenesis via the TGFβ receptor-Smad pathway. | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2017. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 770214 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000024092 | - |
| dc.subject.keyword | Melanogenesis | - |
| dc.subject.keyword | Melanocyte | - |
| dc.subject.keyword | Clusterin | - |
| dc.subject.keyword | Fibroblast-derived factor | - |
| dc.subject.keyword | TGF-β | - |
| dc.description.alternativeAbstract | Clusterin has been known as a biomarker of aging and is highly induced in stressed and senescent cells. In this study, RNA sequencing analysis showed transcripts for clusterin were highly expressed in UV irradiated fibroblasts. A considerable increase of clusterin was detected in the culture medium. We found that the clusterin associates with solar elastosis and is overexpressed in the acutely UV-irradiated skin. To investigate the effect of clusterin on melanogenesis, normal human melanocytes were treated with conditioned media of fibroblasts infected with clusterin-lentivirus or sh-clusterin. It was found that clusterin inhibits melanogenesis through MITF/tyrosinase downregulation via TGF-β signaling. The findings suggest that clusterin inhibits melanogenesis and that it plays a role in controlling UV-induced pigmentary changes. | - |
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- Graduate School of Ajou University > Department of Biomedical Sciences > 3. Theses(Master)
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