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인체 멜라닌 세포에서 Pleiotrophin에 의한 색소 형성 억제 연구
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 강희영 | - |
| dc.contributor.author | 최우종 | - |
| dc.date.accessioned | 2019-10-21T07:23:32Z | - |
| dc.date.available | 2019-10-21T07:23:32Z | - |
| dc.date.issued | 2014-08 | - |
| dc.identifier.other | 17467 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/18477 | - |
| dc.description | 학위논문(석사)--아주대학교 일반대학원 :의생명학과,2014. 8 | - |
| dc.description.tableofcontents | ABSTRACT ⅰ TABLE OF CONTENTS ⅱ LIST OF FIGURES ⅳ,Ⅴ Ⅰ. INTRODUCTION 1 Ⅱ. MATERIAL AND METHODS 5 A. Cell culture 5 B. Ex vivo skin organ culture and pigmentation assay in cultured skin 6 C. Melanin content and tyrosinase activity assays 7 D. Adenovirus production and transduction of cells 7 E. RNA interference 8 F. Semi-quantitative reverse transcriptase PCR and quantitative real-time PCR 8 G. Western blot analysis 10 H. Immunocytochemistry and immunohistochemical analyses of PTN 11 I. Statistical analysis 11 Ⅲ. RESULTS 12 1. PTN is expressed in the melanocytes and fibroblasts of the skin 12 2. PTN inhibits melanin synthesis and the expression of melanogenic enzymes in melanocytes 17 3. PTN inhibits melanin synthesis and the expression of melanogenic enzymes in melanocytes 29 4. PTN induces MITF degradation via Erk1/2 activation 34 Ⅳ. DISCUSSION 40 REFERENCES 45 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 인체 멜라닌 세포에서 Pleiotrophin에 의한 색소 형성 억제 연구 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2014. 8 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 652737 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000017467 | - |
| dc.subject.keyword | 멜라닌세포 | - |
| dc.subject.keyword | 색소형성억제 | - |
| dc.description.alternativeAbstract | Pleiotrophin (PTN) is a secreted heparin-binding protein that is involved in various biological functions of cell growth and differentiation. Little is known about the effects of PTN on the melanocyte function and skin pigmentation. In this study, we investigated whether PTN would affect melanogenesis. PTN was expressed in melanocytes and fibroblasts of human skin. Transfection studies revealed that PTN decreased melanogenesis, probably through MITF degradation via Erk1/2 activation in melanocytes. The inhibitory action of PTN in pigmentation was further confirmed in ex vivo cultured skin and in the melanocytes co-cultured with fibroblasts. These findings suggest that PTN is a crucial factor for the regulation of melanogenesis in the skin. | - |
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- Graduate School of Ajou University > Department of Biomedical Sciences > 3. Theses(Master)
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