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Interferon-gamma suppresses melanogenesis in B16 melanoma cells and primary human melanocytes
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 주일로 | - |
| dc.contributor.author | Son, Jinyoung | - |
| dc.date.accessioned | 2019-10-21T07:19:49Z | - |
| dc.date.available | 2019-10-21T07:19:49Z | - |
| dc.date.issued | 2013-02 | - |
| dc.identifier.other | 13983 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/18193 | - |
| dc.description | 학위논문(석사)아주대학교 일반대학원 :의생명학과,2013. 2 | - |
| dc.description.tableofcontents | TABLE OF CONTENTS ABSTRACT i TABLE OF CONTENTS ii LIST OF FIGURES v ABBREVIATION vii Ⅰ. INTRODUCTION １ Ⅱ. MATERIALS AND METHODS ５ A. Cell culture ５ B. Reagent ５ C. Tyrosinase activity ６ D. Western immunoblot analysis ６ E. RNA extraction and real-time PCR ７ F. Melanin content ７ G. Electrophoresis mobility shift assays (EMSA) ７ H. Duolink assay ８ Ⅲ. RESULTS ９ A. IFN-γ suppresses tyrosinase expression in B16 melanoma cells ９ B. IFN-γ does not affect degradation of tyrosinase in B16 melanoma cells １１ C. IFN-γ inhibits tyrosinase activity and melanin production in B16 melanoma cells １１ D. IFN-γ suppresses the tyrosinase expression and activity in primary human melanocytes １４ E. IFN-γ suppresses MITF binding ability in B16 melanoma cells １４ F. IFN-γ inhibits MITF expression in B16 melanoma cells １８ G. IFN-γ does not regulate MITF mRNA stability in B16 melanoma cells １８ H. CREB binding on promoter of MITF is decreased by IFN-γ treatment ２１ I. JAK-STAT signaling may be involved in the IFN-γ induced inhibitor of melanogemesis ２１ Ⅳ. DISCUSSION ２８ Ⅴ. CONCLUSION ３５ REFERENCES ３６ 국문요약 ４０ | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | Interferon-gamma suppresses melanogenesis in B16 melanoma cells and primary human melanocytes | - |
| dc.title.alternative | 손진영 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | 손진영 | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2013. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 570847 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000013983 | - |
| dc.subject.keyword | Melanogenesis | - |
| dc.subject.keyword | alpha-MSH | - |
| dc.subject.keyword | IFN-gamma | - |
| dc.subject.keyword | Tyrosinase | - |
| dc.subject.keyword | MITF | - |
| dc.subject.keyword | STAT1 | - |
| dc.subject.keyword | CBP | - |
| dc.subject.keyword | CREB | - |
| dc.description.alternativeAbstract | Inflammatory cytokines are closely related to melanogenesis in melanocytes. Here, I studied whether IFN-γ affects melanogenesis, and if so, what is the underlying mechanism involved in it. Using B16 melanoma cell lines and primary human melanocytes, I demonstrated that alpha-MSH induced tyrosinase expression and activity and TRP-1, TRP-2 expression were suppressed by IFN-γ, which resulted from the inhibition of MITF expression, upstream molecule of tyrosinase. Moreover, the inhibitory effects on MITF expression by IFN-γ occured through the disturbance of CREB binding ability on MITF promoter by reducing the binding of CBP and CREB. These results suggest that IFN-γ decreases melanogenesis through inhibiting the binding of CBP and CREB and block the MITF and tyrosinase expression. | - |
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