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Graduate School of Ajou University Department of Biomedical Sciences 3. Theses(Master)

Epigallocatechin-3-gallate and tolfenamic acid induces apoptosis and growth inhibition in head and neck cancer : Involvement of NSAID activated gene-1

DC Field Value Language
dc.contributor.advisor김철호-
dc.contributor.author강성운-
dc.date.accessioned2019-10-21T07:19:44Z-
dc.date.available2019-10-21T07:19:44Z-
dc.date.issued2013-02-
dc.identifier.other13428-
dc.identifier.urihttps://dspace.ajou.ac.kr/handle/2018.oak/18180-
dc.description학위논문(박사)아주대학교 일반대학원 :의생명학과,2013. 2-
dc.description.tableofcontentsABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES v PART ONE I. INTRODUCTION 1 II. MATERIALS AND METHODS 4 A. Cell line and reagents 4 B. Cell proliferation assay 4 C.Terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling (TUNEL) assay 4 D. Flow cytometric detection of apoptotic cells 5 E. Transfection of NAG-1 cDNA and siRNA 5 F. RT-PCR 5 G. Western blot analysis 6 H. Luciferase reporter assay 6 I. Caspase-3 activity assay 6 J. Animal study 7 K. Statistical analysis 7 III. RESULTS 8 A. Effects of EGCG and other catechins on growth and apoptosis of HNSCC cells 8 B. EGCG induces NAG-1 expression in time- and dose-dependent manner in HNSCC cells 10 C. Overexpression of NAG-1 promotes EGCG-induced apoptosis 13 D. Suppression of NAG-1 expression by siRNA desensitizes EGCG responses 16 E. EGCG induces NAG-1 expression through ATM-dependent p53 protein 19 F. Effect of EGCG on tumorigenicity and apoptosis in syngenic mouse model 24 IV. DISCUSSION 27 V. CONCLUSION 29 PART TWO I. INTRODUCTION 30 II. MATERIALS AND METHODS 32 A. Cell lines and reagents 32 B. Cell proliferation assay 32 C. Terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end lebeling (TUNEL) assay 32 D. Mitochondrial membrane potential ( MMP ) assay 33 E. Transfection of NAG-1 cDNA and RNA interference ( RNAi ) 33 F. Western blot analysis 33 G. Animal study 34 H. Statistical analysis 34 III. RESULTS 35 A. Induction of NAG-1 and apoptosis in HNSCC cells by various NSAIDs 35 B. Overexpression and suppression of NAG-1 promotes and attenuates TA-induced apoptosis, respectively 42 C. Effect of TA on tumorigenicity and apoptosis in BALB/c nu/nu mice 47 IV. DISCUSSION 50 V. CONCLUSION 53 REFERENCES 54 국문요약 62-
dc.language.isoeng-
dc.publisherThe Graduate School, Ajou University-
dc.rights아주대학교 논문은 저작권에 의해 보호받습니다.-
dc.titleEpigallocatechin-3-gallate and tolfenamic acid induces apoptosis and growth inhibition in head and neck cancer : Involvement of NSAID activated gene-1-
dc.title.alternativeKang Sung Un-
dc.typeThesis-
dc.contributor.affiliation아주대학교 일반대학원-
dc.contributor.alternativeNameKang Sung Un-
dc.contributor.department일반대학원 의생명과학과-
dc.date.awarded2013. 2-
dc.description.degreeMaster-
dc.identifier.localId570811-
dc.identifier.urlhttp://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000013428-
dc.subject.keywordEGCG-
dc.subject.keywordTolfenamic acid-
dc.subject.keywordNAG-1-
dc.subject.keywordp53-
dc.subject.keywordhead and neck cancer-
dc.subject.keywordapoptosis-
dc.subject.keywordATM-
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