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인체 멜라닌 세포의 멜라닌형성에 대한 WIF-1의 효과
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 강희영 | - |
| dc.contributor.author | Kim, Hye ran | - |
| dc.date.accessioned | 2019-10-21T07:18:27Z | - |
| dc.date.available | 2019-10-21T07:18:27Z | - |
| dc.date.issued | 2011-08 | - |
| dc.identifier.other | 11924 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/17995 | - |
| dc.description | 학위논문(석사)아주대학교 일반대학원 :의생명학과,2011. 8 | - |
| dc.description.tableofcontents | TABLE OF CONTENTS ABSTRACT 5 TABLE OF CONTENTS 6 LIST OF FIGURES 8 LIST OF TABLES 9 I. INTRODUCTION 10 A. Human melanocyte development and pigmentati on 10 B. Wnt signaling pathway 12 C. The Wnt family 14 D. The Wnt antagonist 15 E. Melanocyte and Wnt signaling 18 II. MATERIALS AND METHODS 19 A. Human melanocyte culture 19 B. B16 melanoma cell lines culture 19 C. Human fibroblast culture 20 D. Human keratinocyte culture 20 E. Cell growth assay 20 F. Melanin content determinutesation 12 G. Tyrosinase activity assay 21 H. Skin organ culture 21 I. Pigmentation assay in cultured skin 21 J. Immunocytochemistry 22 K. Western blottiong 23 L. WIF-1 transfection 23 M. Reverse transcriptase-polymerase chain reaction (RT-PCR) 24 N. Statistical analysis 24 III. RESULTS 25 A. WIF-1 is expressed in human melanocytes, fibroblasts and keratinocytes 25 B. WIF-1 increases pigmentation of cultured human skin 27 C. WIF-1 treatment enhances pigmentation in B16 melanoma cells 29 D. WIF-1 treatment enhances pigmentation in human melanocytes 32 E. WIF-1 transfection induces pigmentation in B16 melanoma cells 35 F. WIF-1 transfection induces pigmentation in human melanocytes 38 IV. DISCUSSION 41 V. CONCLUSION 42 REFERENCES 43 국문요약 49 LIST OF FIGURES Fig. 1. Melanogenesis 11 Fig. 2. Wnt signaling pathways 13 Fig. 3. WIF-1 is expressed in human melanocytes, fibroblasts and keratinocytes 26 Fig. 4. WIF-1 increases pigmentation of cultured human skin 28 Fig. 5. WIF-1 treatment enhances pigmentation in B16 melanoma cells 30 Fig. 6. WIF-1 treatment enhances pigmentation in human melanocytes 33 Fig. 7. WIF-1 transfection induces pigmentation in B16 melanoma cells 36 Fig. 8. WIF-1 transfection induces pigmentation in human melanocytes 39 LIST OF TABLES Table 1. Wnt antagonist family molecules 17 Table 2. Effects of WIF-1 on proliferation, melanin contents and tyrosinase activity of B16 melanoma cells 31 Table 3. Effects of WIF-1 on proliferation, melanin contents and tyrosinase activity of human melanocytes 34 Table 4. Effects of WIF-1 transfection on proliferation, melanin contents and tyrosinase activity of B16 melanoma cells 37 Table 5 . Effects of WIF-1 transfection on proliferation, melanin contents and tyrosinase activity of human melanocytes 40 | - |
| dc.language.iso | eng | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 인체 멜라닌 세포의 멜라닌형성에 대한 WIF-1의 효과 | - |
| dc.title.alternative | HYE RAN KIM | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.alternativeName | HYE RAN KIM | - |
| dc.contributor.department | 일반대학원 의생명과학과 | - |
| dc.date.awarded | 2011. 8 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 569611 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011924 | - |
| dc.subject.keyword | melanocyte | - |
| dc.subject.keyword | melanogenesis | - |
| dc.subject.keyword | WIF-1 | - |
| dc.subject.keyword | pigmentation | - |
| dc.subject.keyword | Wnt antagonist | - |
| dc.description.alternativeAbstract | - ABSTRACT - Wnt Inhibitory Factor (WIF)-1 Promotes Melanogenesis in Human Melanocytes Background: Wnt inhibitory factor-1 (WIF-1) acts as a suppressor of Wnt antagonists. There is no study available on melanogenesis effect of human melanocytes. Objectives: In this study, we investigated the expression of WIF-1 in human melanocytes and the effects of WIF-1 on melanogenesis. Method: To determinutese whether WIF-1 is expressed in human melanocytes, we used immunocytochemistry, immunhistochemistry, western blot analysis and reverse transcription polymerase chain reaction. The cell proliferation was measured using the coulter counter. The effects on pigmentation were investigated with measurement of melanin contents, tyrosinase activity and its expression. Result: WIF-1 was expressed in melanocytes. WIF-1 inhibited proliferation of human melanocytes in a dose-dependent manner. WIF-1 also appeared to stimulate the melanin synthesis of human melanocytes. This increase in pigmentation was due to stimulation of the tyrosinase activity and increased expression of MITF and tyrosinase. Conclusion: We demonstrated that WIF-1 was expressed in human melanocytes. Our findings suggest that WIF-1 may play a role in melanogenesis. Key Words: melanocyte, melanogenesis, WIF-1, pigmentation, Wnt antagonist | - |
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- Graduate School of Ajou University > Department of Biomedical Sciences > 3. Theses(Master)
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