repository
Special Graduate Schools
Graduate School of Science and Technology
Department of Chemical Engineering and Biotechnology
3. Theses(Master)
대장암에서 MSI와 hMLH1/hMSH2 면역조직화학염색의 상관관계 분석
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.advisor | 유연우 | - |
| dc.contributor.author | 박경희 | - |
| dc.date.accessioned | 2019-10-21T07:14:17Z | - |
| dc.date.available | 2019-10-21T07:14:17Z | - |
| dc.date.issued | 2010-08 | - |
| dc.identifier.other | 11111 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/17617 | - |
| dc.description | 학위논문(석사)--아주대학교 산업대학원 :화학생명공학과,2010. 8 | - |
| dc.description.abstract | 현미부수체의 불안정성 MSI는 대장암의 탐지등 임상에서 여러 가지 이유로 중요하다. 최근 연구에 따르면 hMLH1과 hMSH2 표현의 면역조직화학의 분석이 MSI - H의 대장 종양의 식별을 위한 신속하고 정확한 방법으로 인식되어지고 있다. 본 연구에서는, MSI와 hMLH1, hMSH2의면역조직화학염색의 상관관계를 알아보고자 하였다. 2008년부터 2009년까지 대장암으로 진단받은 후 대장절제술을 시행한 결장직장암 총 120예 중조직의 양이 충분하고 DNA의 보존상태가 양호하여 MSI 검사가 가능한총 83예를 대상으로 현미부수체불안정성 검사와 hMLH1과 hMSH2에 대한 면역조직화학염색을 실시하였다. 결장직장암 총 83예 중 MSI는 11예 (MSI-H 10예, MSI-L 1예)로 빈도는 13.2%였으며 MSS는 72예 (86.8%)였다. hMLH1은 MSS 총 72예 중 71예(98.6%)가 양성, MSI 11예 중 6예 에서 양성으로 hMLH1 발현의 민감도는 98.6%, 특이도 54.5%였다. hMSH2는 MSS 총 72예 중 72예(100%)가 양성, MSI 11예 중 7예가 양성이었다. hMSH2 발현의 민감도는 100%, 특이도 72.7% 이었다. 전체적인 MSI에 대한 민감도는 98.6%, 특이도는 72.7%였다. 따라서 hMLH1 및 hMSH2에 대한 면역조직화학염색이 MSI의 대체검사로서 가치가 있을 것으로 생각한다. | - |
| dc.description.tableofcontents | I. 서 론 ······················································································· 1 1. 연구배경 ·············································································· 1 1-1. 대장암의 형성과정 ······················································ 2 1-2. 염색체 불안정성 ·························································· 2 1-3. 현미부수체의 정의 ······················································· 5 1-4. 현미부수체 불안정성의 정의 ····································· 5 1-5. 유전자 불안정성의 기전 ·············································· 9 1-6. 현미부수체 불안정성의 기전 ····································· 11 1-6-1. 부정합교정유전자의 배선 돌연변이 ·················· 11 1-6-2. CpG island mutator pathway ····························· 12 1-7. MSI와 hMLH1, hMSH2와의 연관성 ························· 14 1-7-1. Mismatch repair 유전자의 기능 ························· 14 1-7-2. MSI와 hMLH1, hMSH2의 상관관계····················· 14 2.연구의 중요성 및 목적 ························································· 15 II. 실험재료 및 방법 ··································································· 19 1. 실험재료 ··············································································· 19 2. 연구방법 ················································································ 19 2-1. DNA 분리 ····································································· 19 2-2. 현미부수체불안정성 검사············································· 20 2-3. hMLH1과 hMSH2에 대한 면역조직화학 염색 ········· 21 2-4. 통계학적 분석 ······························································· 25 III. 결과 ······················································································ 26 1. 현미부수체 불안정성 빈도 ················································ 26 2. hMLH1, hMSH2 발현과 MSI의 상관관계 ······················ 28 IV. 고찰 ······················································································ 30 V. 결론 ······················································································ 32 VI. 참고문헌 ·············································································· 33 | - |
| dc.language.iso | kor | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 대장암에서 MSI와 hMLH1/hMSH2 면역조직화학염색의 상관관계 분석 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 산업대학원 | - |
| dc.contributor.department | 산업대학원 화학생명공학과 | - |
| dc.date.awarded | 2010. 8 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 568734 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000011111 | - |
| dc.subject.keyword | 대장암 | - |
| dc.subject.keyword | MSI | - |
| dc.subject.keyword | hMLH1/hMSH2 | - |
| dc.subject.keyword | 면역조직화학염색 | - |
- Appears in Collections:
- Special Graduate Schools > Graduate School of Science and Technology > Department of Chemical Engineering and Biotechnology > 3. Theses(Master)
- Files in This Item:
- There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
-
- License
- STATISTICS
- Total Visit :3,751,280
- Total Download :1,818
- Today View :28,938
