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Graduate School of Ajou University
Department of Neuroscience and Technology Course
3. Theses(Master)
뇌 손상 자극시 일어나는 Calpain 의존적인 N-Cadherin의 분해현상의 기전과 기능
| DC Field | Value | Language |
|---|---|---|
| dc.contributor.author | Jang, You-Na | - |
| dc.date.accessioned | 2019-10-21T07:13:23Z | - |
| dc.date.available | 2019-10-21T07:13:23Z | - |
| dc.date.issued | 2010-02 | - |
| dc.identifier.other | 10428 | - |
| dc.identifier.uri | https://dspace.ajou.ac.kr/handle/2018.oak/17430 | - |
| dc.description | 학위논문(박사)--아주대학교 일반대학원 :신경과학기술과정,2010. 2 | - |
| dc.description.tableofcontents | ABSTRACT i TABLE OF CONTENTS iii LIST OF FIGURES vi ABBREVIATION viii I. INTRODUCTION 1 A. Cadherins in nervous system. 1 B. Involvement of proteases on N-cadherin cleavage. 3 C. Calpain activation in pathological conditions 5 D. Aims of study 7 1. Involvement of calpain on N-cadherin cleavage 7 2. Physiological consequences of calpain-mediated N-cadherin cleavage. 8 II. MATERIALS AND METHODS 9 A. Materials 9 B. Cell culture 10 1. Pure cortical neurons 10 2. Astrocytes 10 C. Treatment 10 D. In vitro calpain assay 11 E. DNA constructs and transfection 11 F. Western blot analysis 12 G. Subcellular fractionation 13 H. Biotinylation assay 14 I. Immunoprecipitation (IP) 14 J. Immunofluorescence 14 K. Focal cerebral ischemia in vivo model 15 L. Cell dissociation assay 16 M. RT-PCR (Reverse Transcription-Polymerase Chain Reaction) 16 N. Measurement of cell viability 17 O. Statistical analysis 17 III. RESULTS 18 A. NMDA receptor-induced calcium influx increases N-cadherin cleavage. 18 B. Calpain is responsible for N-cadherin cleavage. 22 C. Calpain cleaves intracellular domain of N-cadherin. 29 D. Calpain is responsible for N-cadherin cleavage in ischemic brain injury. 32 E. Identification of the N-cadherin cleavage sites. 36 F. Calpain-mediated cleavage fragments are degraded by proteasome system. 40 G. Alterations of surface N-cadherin expression and N-cadherin/β-catenin interaction by calpain after NMDA stimulation. 43 H. Nuclear β-catenin accumulation after calpain inhibition induces the expression of Wnt target genes. 48 I. Expression and cleavage of N-cadherin regulates directly AKT signaling. 51 J. Calpain-mediated N-cadherin cleavage inhibits cell-cell adhesion. 53 IV. DISCUSSION 58 REFERENCES 63 국문요약 79 | - |
| dc.language.iso | kor | - |
| dc.publisher | The Graduate School, Ajou University | - |
| dc.rights | 아주대학교 논문은 저작권에 의해 보호받습니다. | - |
| dc.title | 뇌 손상 자극시 일어나는 Calpain 의존적인 N-Cadherin의 분해현상의 기전과 기능 | - |
| dc.type | Thesis | - |
| dc.contributor.affiliation | 아주대학교 일반대학원 | - |
| dc.contributor.department | 일반대학원 신경과학기술과정 | - |
| dc.date.awarded | 2010. 2 | - |
| dc.description.degree | Master | - |
| dc.identifier.localId | 568444 | - |
| dc.identifier.url | http://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010428 | - |
| dc.subject.keyword | Calpain-Mediated N-Cadherin Proteolytic | - |
| dc.subject.keyword | 뇌 손상 자극 | - |
| dc.subject.keyword | N-Cadherin의 분해현상 | - |
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