백스 유전자 조작 생쥐를 이용한 해마 치상의 공간정보 처리 메커니즘에 대한 연구

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dc.contributor.advisor정민환-
dc.contributor.authorLee, Jong Won-
dc.date.accessioned2019-10-21T07:13:21Z-
dc.date.available2019-10-21T07:13:21Z-
dc.date.issued2010-02-
dc.identifier.other10413-
dc.identifier.urihttps://dspace.ajou.ac.kr/handle/2018.oak/17423-
dc.description학위논문(박사)--아주대학교 일반대학원 :신경과학기술과정,2010. 2-
dc.description.abstract인간을 비롯한 동물들은 외부 시각 단서와 자신의 움직임에 근거하여 외부 공간을 표상한다는 것이 잘 알려져 있다. 그러나 다양한 감각 정보들이 어떻게 통합되어 외부 공간을 표상하는지는 잘 알려져 있지 않다. 이러한 과정에 해마 치상이 어떻게 관련되어 있는지 알아보기 위해, 치상 선택적 손상 모델인 백스 유전자 결손 생쥐를 이용해 신경 생리학적 실험과 행동 실험을 수행하였다. 그 결과 백스 유전자 결손 생쥐에서 해마 신경세포의 공간 발화율이 외부 단서와는 완전히 분리되어 발화하는 양상을 보였고, 외부 시각 단서보다는 노출된 전체 공간에 대하여 발화 양상을 유지하려는 경향을 보였다. 또한 전통적 공간 학습 과제에서는 대조군과 마찬가지로 학습을 잘 하나, 자신의 움직임에 근거한 정보 (추측 항법, dead reckoning) 와 외부 시각단서가 불일치했을 때 외부 시각단서에 근거한 공간 학습에서는 학습이 저해되었다. 이러한 결과들은 해마 치상이 외부 시각 단서를 내부 공간 표상에 통합하는데 있어서 역할을 한다는 것을 시사해준다. 또한 백스 유전자 결핍 생쥐는 이전에 방문했던 장소와 방문하지 않았던 장소를 구분하는 과제도 잘 수행하지 못하였는데, 이것은 해마 치상이 단지 시각 단서 뿐만 아니라 물체나 사건 (objects ,events) 등의 감각 정보를 내부 공간 정보에 연합 시키는데도 역할을 할 가능성이 높다는 것을 시사해 준다.-
dc.description.tableofcontentsABSTRACT ⅰ TABLE OF CONTENTS ⅲ LIST OF FIGURES ⅴ Ⅰ. INTRODUCTION 1 A. Spatial representation in the hippocampus 1 B. Dissociated streams of inputs into hippocampus 2 C. The role of distinct hippocampal subregions 5 1. DG 5 2. CA3 6 3. CA1 7 D. Bax knock-out mouse with impaired adult neurogenesis 8 Ⅱ. MATERIALS AND METHODS 11 A. NEUROPHYSIOLOGY 11 1. Animals 11 2. Behavioral paradigm 11 3. Unit recording 14 4. Analysis 15 5. Statistical analysis 16 6. Histology 18 B. BEHAVIOR 20 1. Spatial reference memory task in water maze 20 2. Spatial reference memory task on radial maze 20 3. Spatial working memory task on radial maze 21 4. Statistical analysis 22 Ⅲ. RESULTS 23 A. NEUROPHYSIOLOGY 23 1. Running speed 23 2. Theta EEG for control and Bax-KO mice 23 3. Characteristics of unit recording 24 4. Dependence of spatial firing on sensory cue 27 5. Relationship between spatial selectivity and cue-dependent firing 33 6. Similar rate remapping scores 36 B. BEHAVIOR 38 1. Performance in water maze 38 2. Performance in radial maze spatial reference memory task 40 3. Performance in spatial working memory task 43 Ⅳ. DISCUSSION 46 A. Role of DG in aligning internal spatial map to external landmark 46 B. Role of CA3 versus DG in aligning internal spatial map to external landmark 49 C. Strong influence of trisynaptic pathway on discharges of CA1 neurons 51 D. Role of DG in binding spatial and non-spatial information 52 Ⅴ. CONCLUSIONS 54 REFERENCES 55 국문요약 69-
dc.language.isoeng-
dc.publisherThe Graduate School, Ajou University-
dc.rights아주대학교 논문은 저작권에 의해 보호받습니다.-
dc.title백스 유전자 조작 생쥐를 이용한 해마 치상의 공간정보 처리 메커니즘에 대한 연구-
dc.title.alternativeJong Won Lee-
dc.typeThesis-
dc.contributor.affiliation아주대학교 일반대학원-
dc.contributor.alternativeNameJong Won Lee-
dc.contributor.department일반대학원 신경과학기술과정-
dc.date.awarded2010. 2-
dc.description.degreeMaster-
dc.identifier.localId568435-
dc.identifier.urlhttp://dcoll.ajou.ac.kr:9080/dcollection/jsp/common/DcLoOrgPer.jsp?sItemId=000000010413-
dc.subject.keywordhippocampus-
dc.subject.keyworddentate gyrus-
dc.subject.keywordlearning and memory-
dc.subject.keywordplace cells-
dc.subject.keywordspatial memory-
dc.subject.keyworddead reckoning-
dc.description.alternativeAbstractIt is well known that humans and animals form internal representations of external space based on their own body movement (dead reckoning) as well as external landmarks. It is poorly understood, however, how different types of information are integrated to form a unified representation of external space (i.e., cognitive map). To examine the role of dentate gyrus in this process, physiological and behavioral experiments were conducted using Bax knock-out (Bax-KO) mice in which newly generated granule cells continue to accumulate disrupting neural circuitry specifically in the dentate gyrus. Unlike Wild-type (WT) littermates, spatial firing of hippocampal neurons is completely dissociated from a distinct visual cue and instead, tended to stay constant relative to the recording room in Bax-KO mice. Moreover, whereas spatial learning was intact in Bax-KO mice in conventional spatial reference memory tasks, they were impaired in finding a target location based on visual landmarks when target locations predicted by dead reckoning and visual landmarks were made incongruent. These results suggest that the dentate gyrus is involved in aligning animal’s internal spatial representation to external landmarks, which is a necessary process for representing a distinct spatial map for each environment (i.e., remembering ‘where’). Bax-KO mice were also impaired in discriminating between previously visited and unvisited locations, which suggests a broader role of dentate gyrus in integrating different sources of information. Collectively, these results raise the possibility that the DG might be a region where the internal spatial representation is rapidly associated with the sensory information on external objects and events.-
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Graduate School of Ajou University > Department of Neuroscience and Technology Course > 3. Theses(Master)
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